Genomic location of Gb1, a unique gene conferring wheat resistance to greenbug biotype F  

作　　者：Xiangyang Xu Genqiao Li Guihua Bai Brett FCarver Ruolin Bian Amy Bernardo JScott Armstrong 

机构地区：[1]USDA-ARS Wheat,Peanut,and Other Field Crop Research Unit,Stillwater,OK 74075,USA [2]USDA-ARS Hard Winter Wheat Genetics Research Unit,Manhattan,KS 66506,USA [3]Department of Agronomy,Kansas State University,Manhattan,KS 66506,USA [4]Department of Plant and Soil Sciences,Oklahoma State University,Stillwater,OK 74078,USA

出　　处：《The Crop Journal》2023年第5期1595-1599,共5页作物学报（英文版）

基　　金：supported by the USDA ARS CRIS Project (3072– 21000–009–00D)。

摘　　要：Greenbug(Schizaphis graminum, Rondani) is a serious insect pest in many wheat growing regions and has been infesting cereal crops in the USA for over a century. Continuous occurrence of new greenbug biotypes makes it essential to explore all greenbug resistant sources available to manage this pest. Gb1, a recessive greenbug resistance gene in DS28A, confers resistance to several economically important greenbug biotypes and is the only gene found to be resistant to greenbug biotype F. A set of 174 F_(2:3)lines from the cross DS28A × Custer was evaluated for resistance to greenbug biotype F in 2020 and 2022. Selective genotyping of the corresponding F_(2) population using single nucleotide polymorphism(SNP) markers generated by genotyping-by-sequencing(GBS) led to the identification of a candidate genomic region for Gb1. Thus, SSR markers previously mapped in this region were used to genotype the entire F2population,and kompetitive allele specific PCR(KASP) markers were also developed from SNPs in the target region.Gb1 was placed in the terminal region of the short arm of chromosome 1A, and its location was confirmed in a second population derived from the cross DS28A × PI 697274. The combined data analysis from the two mapping populations delimited Gb1 to a < 1 Mb interval between 13,328,200 and 14,241,426 bp on1AS.

关 键 词：Wheat Greenbug resistance gene Gb1 KASP markers Linkage analysis Genotyping-by-sequencing 

分 类 号：S512.1[农业科学—作物学]