Identification of candidate genes for aphid resistance in upland cotton by QTL mapping and expression analysis  

作　　者：Qiushuang An Zhenyuan Pan Nurimanguli Aini Peng Han Yuanlong Wu Chunyuan You Xinhui Nie 

机构地区：[1]Key Laboratory of Oasis Ecology Agricultural of Xinjiang Production and Construction Corps,Agricultural College,Shihezi University,Shihezi 832003,Xinjiang,China [2]Cotton Research Institute of the Shihezi Academy of Agriculture Science,Shihezi 832011,Xinjiang,China

出　　处：《The Crop Journal》2023年第5期1600-1604,共5页作物学报（英文版）

基　　金：supported by the Corps Science and Technology Innovation Talent Plan (2021CB028);the Shihezi Science and Technology Research Key Field Science and Technology Research Project (2022NY01);the Fifth Division Science and Technology Plan Project (2021NY02);the Young and Middle-aged Leading Talent Plan (2020CB017)。

摘　　要：Lignin is one of the main components of cell walls and is essential for resistance to insect pests in plants.Cotton plants are damaged by aphid(Aphis gossypii) worldwide but resistant breeding is undeveloped due to scarce knowledge on resistance genes and the mechanism. This study reported a lignin biosynthesisrelated gene identified in the F_(2) population derived from the cross between cotton cultivars Xinluzao 61(resistant to aphid) and Xinluzao 50(susceptible to aphid). A quantitative trait locus was mapped on chromosome D04 with a logarithm of odds(LOD) score of 5.99 and phenotypic effect of 27%. RNA-seq analysis of candidate intervals showed that the expression level of GH_D04G1418 was higher in the resistant cultivar than in the susceptible cultivar. This locus is close to AtLAC4 in the phylogenetic tree and contains a conserved laccase domain. Hence, it was designated GhLAC4-3. Silencing of GhLAC4-3 in Xinluzao 61 via virus-induced gene silencing(VIGS) resulted in decreased lignin content and increased susceptibility to aphids. These results suggest that GhLAC4-3 might enhance aphid resistance by regulating lignin biosynthesis in cotton.

关 键 词：GhLAC4-3 LIGNIN Gossypium hirsutum Aphid resistance 

分 类 号：S562[农业科学—作物学]