全自动妇科分泌物分析系统在阴道微生态评价中的应用效果分析  

Analysis on application effect of automatic gynecological secretion analysis system in vaginal microecology evaluation

作　　者：黄宇飞 马芬 刘琛 张咪咪 HUANG Yufei;MA Fen;LIU Chen;ZHANG Mimi(Department of Clinical Laboratory,Guangming District Maternal and Child Health Care Hospital,Shenzhen,Guangdong 518107,China;Department of Clinical Laboratory,Shenzhen(Guangming)Hospital,University of Chinese Academy of Sciences,Shenzhen,Guangdong 518107,China)

机构地区：[1]广东省深圳市光明区妇幼保健院检验科,广东深圳518107 [2]中国科学院大学深圳医院(光明)检验科,广东深圳518107

出　　处：《检验医学与临床》2023年第19期2798-2802,共5页Laboratory Medicine and Clinic

基　　金：广东省深圳市光明区软科学研究项目(2021R01087)。

摘　　要：目的探讨全自动妇科分泌物分析系统在阴道微生态评价中的应用效果。方法选取阴道炎患者307例,采用革兰染色镜检、湿片镜检和全自动妇科分泌物分析系统(迪瑞GMD-S600)对阴道清洁度、病原微生物进行检测并比较。以革兰染色镜检结果为金标准,比较全自动妇科分泌物分析系统和湿片镜检对外阴真菌性阴道炎、细菌性阴道炎(BV)、滴虫性阴道炎的诊断效能。结果全自动妇科分泌物分析系统、革兰染色镜检和湿片镜检的阴道清洁度阳性率及白细胞、念珠菌、线索细胞、乳酸杆菌检出率比较,差异均无统计学意义(P>0.05),而对滴虫检出率比较,差异有统计学意义(P<0.05)。与湿片镜检比较,全自动妇科分泌物分析系统在BV诊断中,灵敏度较高、符合率较高、阴性预测值较高、漏诊率较低;与湿片镜检比较,在外阴真菌性阴道炎诊断中全自动妇科分泌物分析系统灵敏度较高、符合率较高、阴性预测值较高、漏诊率较低;与湿片镜检比较,在滴虫性阴道炎诊断中全自动妇科分泌物分析系统特异度较低、符合率较低、误诊率较高。结论全自动妇科分泌物分析系统通过形态学和功能学互补作用,综合评价阴道微生态性能良好,但对滴虫诊断的准确率比镜检低。当形态学结果和功能学结果不一致时,需要人工复检。Objective To explore the application effect of the automatic gynecological secretion analysis system in the vaginal microecological evaluation.Methods A total of 307 patients with vaginitis were selected.The vaginal cleanliness and pathogenic microorganisms were detected and compared by the Gram staining microscopic examination,wet slide microscopic examination and the automatic gynecological secretion analysis system(Dirui GMD-S600).The results of Gram staining microscopic examination were used as the gold standard to compare the diagnostic efficiency of the automatic gynecological secretion analysis system and wet microscopic examination in vulvovaginal colpomycosis,bacterial vaginosis(BV)and trichomonas vaginitis.Results There was no statistically significant difference in the positive rate of vaginal cleanliness,the detection rates of leukocyte,candida,clue cell and Lactobacillus among the automatic gynecological secretion analysis system,Gram staining microscopic examination and wet microscopic examination(P>0.05).There was statistically significant difference in the detection rate of trichomonas(P<0.05).Compared with the wet slide microscopic examination,the automatic gynecological secretion analysis system has higher sensitivity in the diagnosis of BV,higher coincidence rate,higher negative predictive value and lower missed diagnosis rate;compared with the wet slide microscopic examination in the diagnosis of vulvovaginal colpomycosis,the automatic gynecological secretion analysis system had higher sensitivity,higher coincidence rate,higher negative predictive value and lower missed diagnosis rate;compared with the wet slide microscopic examination in the diagnosis of trichomonal vaginitis,the automatic gynecological secretion analysis system had the lower specificity,lower coincidence rate and higher misdiagnosis rate.Conclusion The automatic gynecological secretion analysis system has good performance in comprehensively evaluating the vaginal microecology through the complementary role of morphology and

关 键 词：全自动妇科分泌物分析系统 湿片镜检 革兰染色镜检 

分 类 号：R711.31[医药卫生—妇产科学]