Fusarium-produced vitamin B6 promotes the evasion of soybean resistance by Phytophthora sojae  

作　　者：Shuchen Wang Xiaoyi Zhang Zhichao Zhang Yun Chen Qing Tian Dandan Zeng Miao Xu Yan Wang Suomeng Dong Zhonghua Ma Yuanchao Wang Xiaobo Zheng Wenwu Ye 

机构地区：[1]Department of Plant Pathology,Nanjing Agricultural University,Nanjing 210095,China [2]Key Laboratory of Plant Immunity,Nanjing Agricultural University,Nanjing 210095,China [3]Key Laboratory of Soybean Disease and Pest Control(Ministry of Agriculture and Rural Affairs),Nanjing Agricultural University,Nanjing 210095,China [4]Key Laboratory of Molecular Biology of Crop Pathogens and Insects,Institute of Biotechnology,Zhejiang University,Hangzhou 310058,China

出　　处：《Journal of Integrative Plant Biology》2023年第9期2204-2217,共14页植物学报（英文版）

基　　金：supported by grants from the National Natural Science Foundation of China (32172374;31721004);the China Agriculture Research System (CARS-004-PS14)。

摘　　要：Plants can be infected by multiple pathogens concurrently in natural systems. However,pathogen–pathogen interactions have rarely been studied. In addition to the oomycete Phytophthora sojae, fungi such as Fusarium spp. also cause soybean root rot. In a 3-year field investigation, we discovered that P. sojae and Fusarium spp. frequently coexisted in diseased soybean roots. Out of 336 P. sojae–soybean–Fusarium combinations,more than 80% aggravated disease. Different Fusarium species all enhanced P. sojae infection when co-inoculated on soybean. Treatment with Fusarium secreted non-proteinaceous metabolites had an effect equal to the direct pathogen coinoculation. By screening a Fusarium graminearum mutant library, we identified Fusarium promoting factor of Phytophthora sojae infection 1(Fpp1),encoding a zinc alcohol dehydrogenase. Fpp1 is functionally conserved in Fusarium and contributes to metabolite-mediated infection promotion, in which vitamin B6(VB6) produced by Fusarium is key. Transcriptional and functional analyses revealed that Fpp1 regulates two VB6 metabolism genes, and VB6 suppresses expression of soybean disease resistance-related genes. These results reveal that co-infection with Fusarium promotes loss of P. sojae resistance in soybean, information that will inform the sustainable use of diseaseresistant crop varieties and provide new strategies to control soybean root rot.

关 键 词：fungal metabolites pathogen–host interaction Phytophthora sojae soybean root rot vitamin B6 

分 类 号：S435.651[农业科学—农业昆虫与害虫防治]