基于指纹图谱结合化学计量学评价栽培与野生五指毛桃质量  

Evaluation of Cultivated Fici Simplicissimae Radix and Wild Fici Simplicissimae Radix by Fingerprints and Chemometrics

作　　者：杨小催 郭凯欣 李燕芳 唐慧[2] 张庆业[3] 潘海华 郑丽思 柯雪红[3] YANG Xiaocui;GUO Kaixin;LI Yanfang;TANG Hui;ZHANG Qingye;PAN Haihua;ZHENG Lisi;KE Xuehong(Qingyuan Hospital of Traditional Chinese Medicine,Qingyuan,Guangdong,China 511500;Guangzhou University of Traditional Chinese Medicine,Guangzhou,Guangdong,China 510405;The First Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine,Guangzhou,Guangdong,China 510405)

机构地区：[1]广东省清远市中医院,广东清远511500 [2]广州中医药大学,广东广州510405 [3]广州中医药大学第一附属医院,广东广州510405

出　　处：《中国药业》2023年第19期63-69,共7页China Pharmaceuticals

基　　金：广东省中医药局科研项目[20201404];广东省清远市科技计划项目[2021SJXM025]。

摘　　要：目的 建立五指毛桃的高效液相色谱(HPLC)指纹图谱,结合化学计量学方法确定野生、栽培五指毛桃的质量差异标志物。方法 色谱柱为Hypersil ODS2柱(250 mm×4.6 mm,5μm),流动相为乙腈-水(梯度洗脱),流速为1.0 mL/min,检测波长为320 nm,柱温为30℃,进样量为20μL。对10批栽培五指毛桃与21批野生五指毛桃样品进行分析,建立HPLC指纹图谱,采用中药色谱指纹图谱相似度评价系统(2004A版)进行相似度评价,确认共有峰,通过与对照品比对指认共有峰,并结合化学计量学方法对野生、栽培五指毛桃的质量及其控制方法进行评价。结果 建立了31批五指毛桃样品的HPLC指纹图谱,共标定19个共有峰,以10批栽培五指毛桃指纹图谱的共有模式作为五指毛桃标准指纹图谱,经与对照品比对指认了其中3个差异性成分,分别为补骨脂素、芹菜素和5-甲氧基补骨脂素;以栽培五指毛桃的共有模式为对照图谱,10批栽培五指毛桃及21批野生五指毛桃的相似度分别为0.847~0.947和0.503~0.594;层次聚类分析和主成分分析将31批五指毛桃基本分为栽培和野生五指毛桃;偏最小二乘法-判别分析筛选得到6个差异标志物,经与对照品比对指认了其中3个差异性成分,分别为芹菜素、补骨脂素、5-甲氧基补骨脂素。栽培五指毛桃的芹菜素含量为每100 g药材含0.007 7 g,高于野生五指毛桃的0.000 8 g。结论 所建立的指纹图谱合理、有效、准确,结合化学计量学方法表征信息更全面,可为五指毛桃的质量控制与品质评价提供参考。Objective To establish a high-performance liquid chromatography(HPLC) fingerprints of Fici Simplicissimae Radix,and to determine the biomarkers for quality differences between wild Fici Simplicissimae Radix and cultivated Fici Simplicissimae Radix by fingerprint and chemometrics.Methods The chromatographic column was Hypersil ODS2 column(250 mm × 4.6 mm,5 μm),the mobile phase was acetonitrile-water(gradient elution),the flow rate was 1.0 mL/min,the detection wavelength was 320 nm,the column temperature was 30 ℃,and the injection volume was 20 μL.The samples of 10 batches of cultivated Fici Simplicissimae Radix and 21 batches of wild Fici Simplicissimae Radix were analyzed to establish HPLC fingerprints.The Similarity Evaluation System of Chromatographic Fingerprint of Traditional Chinese Medicine(2004A) was used to evaluate the similarity and identify the common peak.The common peak was identified by comparison of reference substances,and the quality and control methods of wild Fici Simplicissimae Radix and cultivated Fici Simplicissimae Radix were analyzed and evaluated by fingerprints and chemometrics.Results The HPLC fingerprints of 31 batches of Fici Simplicissimae Radix samples were established,with a total of 19 common peaks calibrated.The common pattern of 10 batches of cultivated Fici Simplicissimae Radix was taken as the standard fingerprint of Fici Simplicissimae Radix,and three different components were identified by the reference substance,namely psoralen,apigenin and 5-methoxypsoralen.With the common pattern of cultivated Fici Simplicissimae Radix as the reference chromatograms,the similarities of 10 batches of cultivated Fici Simplicissimae Radix and 21 batches of wild Fici Simplicissimae Radix were 0.847-0.947 and 0.503-0.594,respectively.According to cluster analysis and principal component analysis(PCA),31 batches of Fici Simplicissimae Radix were divided into cultivated Fici Simplicissimae Radix and wild Fici Simplicissimae Radix.Six differential markers were screened by partial least squar

关 键 词：五指毛桃 栽培 野生 高效液相色谱法 指纹图谱 化学计量学 质量评价 

分 类 号：R932[医药卫生—生药学] R284.1[医药卫生—药学] R286.0