电针神庭、百会穴对脑缺血再灌注后学习记忆障碍大鼠皮质区内LC3 Ⅱ和Beclin-1的影响  

Effects of electroacupuncture Shenting and Baihui Points on LC3 Ⅱ and Beclin-1 in cerebral cortex of rats with learning and memory impairment after cerebral ischemia reperfusion

作　　者：黄金 王欣雨[1] 高玲莉[1] 冯晓东[1,2] HUANG Jin;WANG Xin-Yu;GAO Ling-Li(Henan University of Chinese Medicine,Zhengzhou 450000,Henan,China)

机构地区：[1]河南中医药大学,河南郑州450000 [2]河南中医药大学第一附属医院

出　　处：《中国老年学杂志》2023年第19期4752-4757,共6页Chinese Journal of Gerontology

基　　金：国家自然科学基金(No.81574042);河南科技攻关计划(No.202102310167);河南省中医药科研专项(No.2018JDZX011)。

摘　　要：目的 观察大脑中动脉缺血再灌注(MCAO)模型大鼠皮质区中自噬体的形成。方法 线栓法制备局灶性脑缺血损伤大鼠模型。45只SD大鼠造模成功后用随机数字表法分为电针组、模型组和假手术组各15只。电针组行电针干预治疗14 d。透射电镜下观察大鼠皮质区溶酶体及自噬体的形成;Western印迹及实时荧光定量聚合酶链反应(RT-PCR)检测大鼠脑组织中酵母Atg6基因在哺乳动物中同源类似物(Beclin)-1和自噬微管相关蛋白轻链3抗体(LC3)Ⅱ含量。结果 电针组溶酶体及自噬体形成数量明显多于模型组和假手术组(P<0.05);与模型组相比,电针组脑组织中Beclin-1和LC3Ⅱ含量明显升高(P<0.05);模型组脑组织中Beclin-1和LC3Ⅱ含量显著高于假手术组(P<0.05)。结论 自噬体形成及自噬相关蛋白可以调控脑缺血再灌注后大鼠的学习及记忆能力,自噬网络及其调控机制在脑卒中所导致的认知障碍形成及恢复过程中起着重要作用。Objective To observe the formation of autophagy in cortical region of middle cerebral artery ischemia reperfusion(MCAO)rat models.Methods Rat models of focal cerebral ischemia injury were prepared by thread embolism.45 SD rats were randomly divided into electroacupuncture group,model group and sham operation group after the models were succeeded,with 15 rats in each group.The electroacupuncture group was given received electroacupuncture intervention for 14 d.The formation of lysosomes and autophagy in rat cortex was observed under transmission electron microscope.The contents of homologous analogues of yeast Atg6 gene in mammals(Beclin)-1 and autophagy microtubule-associated protein light chain 3 antibody(LC3)Ⅱin rat brains were detected by Western blot and real-time fluorescence polymerse chair reaction(RT-PCR).Results The number of lysosomes and autophagy in the electroacupuncture group were significantly higher than those in the model group and the sham operation group(P<0.05).Compared with the model group,the contents of Beclin1 and LC3Ⅱwere significantly increased in the brains of the electroacupuncture group(P<0.05).The contents of Beclin1 and LC3Ⅱin the brains of the model group were higher than those in the sham operation group(P<0.05).Conclusions The formation of autophagy and its associated proteins could regulate the learning and memory ability of rats after cerebral ischemia reperfusion,autophagy networks and their regulatory mechanisms play an important role in the formation and recovery of cognitive impairment caused by stroke.

关 键 词：电针 透射电镜 脑缺血 自噬体 

分 类 号：R277[医药卫生—中医学]