加味大黄[庶虫]虫丸对原发性胆汁性胆管炎肝星状细胞损伤状态下TGF-β1、Smads的影响  

Effect of Jiawei Dahuang Zhechong pills on transforming growth factor-β1 and Smads under the status of hepatic stellate cell injury in primary biliary cholangitis

作　　者：熊小花 柳玉佳[1] 杨柳 王莘智[1] XIONG Xiaohua;LIU Yujia;YANG Liu;WANG Shenzhi(The First Affiliated Hospital of Hunan University of Chinese Medicine,Changsha 410007,Hunan,China)

机构地区：[1]湖南中医药大学第一附属医院,湖南长沙410007

出　　处：《湖南中医杂志》2023年第8期187-191,共5页Hunan Journal of Traditional Chinese Medicine

基　　金：湖南省自然科学基金项目(2020JJ4479)。

摘　　要：目的:观察加味大黄[庶虫]虫丸对原发性胆汁性胆管炎(PBC)肝星状细胞损伤状态下转化生长因子-β1(TGF-β1)、Smads的影响。方法:将20只大鼠随机分为干预组与对照组,每组各10只。干预组灌胃加味大黄[庶虫]虫丸混悬液1.5 ml/100 g,对照组灌胃等量0.9%氯化钠注射液,每天1次,持续1周。将肝星状细胞T6(HSC-T6)细胞分为6组,分别为空白组、对照组、5%空白血清组、10%空白血清组、5%含药血清组、10%含药血清组,培养24 h。建立乳酸损伤模型,造模成功后分为5组:空白组、对照组、正常组、模型组、中药组。测定细胞活性,细胞中TGF-β1、Smad3、Smad7 mRNA表达,TGF-β1、磷酸化Smad3、磷酸化Smad7 mRNA蛋白表达。结果:各组HSC-T6细胞活性对照组、5%空白血清组、10%空白血清组、5%含药血清组、10%含药血清组与空白组比较,差异均有统计学意义(P<0.01)。TGF-β1、Smad3、Smad7 mRNA表达及TGF-β1、p-Smad3、p-Smad7 mRNA蛋白表达水平,模型组与空白组比较,中药组与模型组比较,差异均有统计学意义(P<0.01或P<0.05)。结论:加味大黄[庶虫]虫丸治疗PBC的作用机制与降低TGF-β1、Smad3水平,提高Smad7水平相关。Objective:To investigate the effect of Jiawei Dahuang Zhechong pills on transforming growth factor-β1(TGF-β1)and Smads under the status of hepatic stellate cell injury in primary biliary cholangitis(PBC).Methods:A total of 20 rats were randomly divided into intervention group and control group,with 10 rats in each group.The rats in the intervention group were given the suspension of Jiawei Dahuang Zhechong pills by gavage,and those in the control group were given an equal volume of 0.9%sodium chloride injection by gavage,once a day for one week.Hepatic stellate cell-T6(HSC-T6)cells were divided into blank group,control group,5%blank serum group,10%blank serum group,5%drug-containing serum group,and 10%drug-containing serum group,and the cells were cultured for 24 hours.A model of lactic acid-induced injury was established,and after successful modeling,the experiment was divided into blank group,control group,normal group,model group,and traditional Chinese medicine(TCM)group.Cell viability was measured,as well as the mRNA expression lev-els of TGF-β1,Smad3,and Smad7 and the mRNA and protein expression levels of TGF-β1,phosphorylated Smad3(p-Smad3),and phosphorylated Smad7(p-Smad7)in cells.Results:There was a significant difference in the viability of HSC-T6 cells between the blank group and the other five groups(control group,5%blank serum group,10%blank serum group,5%drug-containing serum group,and 10%drug-containing serum group)(P<0.01).There were significant differences in the mRNA expression levels of TGF-β1,Smad3,and Smad7 and the mRNA and protein expression levels of TGF-β1,p-Smad3,and p-Smad7 between the model group and the blank group and between the TCM group and the model group(P<0.01 or P<0.05).Conclusion:Jiawei Dahuang Zhechong pills exert a therapeutic effect on PBC by reducing the levels of TGF-β1 and Smad3 and increasing the level of Smad7.

关 键 词：原发性胆汁性胆管炎 肝星状细胞 损伤状态 加味大黄[庶虫]虫丸 TGF-β1 SMADS 

分 类 号：R285.5[医药卫生—中药学]