一株好氧反硝化菌的脱氮特性及其氮代谢机理研究  

Nitrogen removal characteristics and metabolism mechanism of an aerobic denitrifying bacteria

作　　者：杨景瑞 王莹[2] 陈虎 吕永康[2] YANG Jingrui;WANG Ying;CHEN Hu;LÜYongkang(Department of Resources and Environmental Engineering,Shanxi Institute of Energy,Taiyuan 030000,China;State Key Laboratory of Clean and Efficient Coal Utilization,Taiyuan University of Technology,Taiyuan 030024,China;College of Environmental Science and Engineering,Taiyuan University of Technology,Taiyuan 030024,China)

机构地区：[1]山西能源学院资源与环境工程系,山西太原030000 [2]太原理工大学省部共建煤基能源清洁高效利用国家重点实验室,山西太原030024 [3]太原理工大学环境科学与工程学院,山西太原030024

出　　处：《工业水处理》2023年第10期135-141,共7页Industrial Water Treatment

基　　金：山西省基础研究计划项目(202203021212474);山西省高等学校科技创新项目(2022L606)。

摘　　要：以蒸氨废水中筛选获得的高效好氧反硝化菌假单胞菌(Pseudomonas sp.LV2)为研究对象,系统研究了LV2菌株的好氧反硝化特性,以及不同碳源、培养温度、C/N、转速等因素对菌株脱氮效果的影响,并通过反硝化关键酶活性测定、气体检测及氮平衡实验推测菌株的好氧反硝化路径。结果表明,LV2菌株表现出高效的NO_(3)^(-)-N去除能力,24 h时NO_(3)^(-)-N去除率可达到98%以上;LV2菌株最佳脱氮条件为以丙酮酸钠为碳源,温度30℃,C/N=16,转速120 r/min。在LV2菌株脱氮过程中检测到气态产物N_(2),并检测出反硝化过程中的关键酶硝酸盐还原酶(NR)和亚硝酸盐还原酶(NIR),推测部分NO_(3)^(-)-N作为营养物质被直接吸收到细胞中,部分NO_(3)^(-)-N则通过好氧反硝化作用转化为N_(2)。好氧反硝化菌LV2在实际废水的NO_(3)^(-)-N污染生物治理方面具有很大的应用前景。Taking the screened highly efficient aerobic denitrifying bacteria Pseudomonas sp.LV2 from ammonia steaming wastewater as the research object,the aerobic denitrification characteristics of strain LV2 were systematically studied,as well as the influences of different carbon sources,culture temperature,C/N,rotational speed and other factors on the denitrification effect.The aerobic denitrification pathway of strain LV2 was deduced by the functional enzymatic activity measurement,gaseous nitrogen product detection and nitrogen balance calculation.The results showed that strain LV2 exhibited an efficient nitrate removal capacity,and the nitrate removal efficiency could reach more than 98%in 24 h.The optimal nitrogen removal conditions of strain LV2 were sodium pyruvate as carbon source,temperature 30℃,C/N=16,and rotational speed 120 r/min.During the denitrification process of strain LV2,gaseous product N_(2) was detected,and the key enzymes required for the denitrification process,nitrate reductase(NR) and nitrite reductase(NIR),were detected.It was inferred that part of nitrate was directly absorbed into cells as nutrients,and some nitrate was converted into N_(2) through aerobic denitrification.Aerobic denitrifying bacteria LV2 had great application prospect in the biological treatment of nitrate pollution in practical wastewater.

关 键 词：假单胞菌 好氧反硝化 影响因素 氮代谢途径 

分 类 号：X703[环境科学与工程—环境工程]