牡蛎相关微病毒科噬菌体基因组的鉴定和进化分析  

Identification and evolutionary analysis of genome of oyster-associated Microviridae

作　　者：刘敏 刘畅 刘广锋[2] 朱鹏 谢科明 姜敬哲[2] LIU Min;LIU Chang;LIU Guangfeng;ZHU Peng;XIE Keming;JIANG Jingzhe(College of Marine Ecology and Environment,Shanghai Ocean University,Shanghai 201306,China;South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences/Key Laboratory of South China Sea Fishery,Resources Exploitation&Utilization,Ministry of Agriculture and Rural Affairs,Guangzhou 510300,China;College of Life Science and Biopharmacy,Guangdong Pharmaceutical University,Guangzhou 510005,China)

机构地区：[1]上海海洋大学海洋生态与环境学院,上海201306 [2]中国水产科学研究院南海水产研究所/农业农村部南海渔业资源开发利用重点实验室,广东广州510300 [3]广东药科大学生命科学与生物制药学院,广东广州510005

出　　处：《南方水产科学》2023年第5期134-142,共9页South China Fisheries Science

基　　金：广东省重点领域研发计划项目(2022B1111030001);国家自然科学基金面上项目(31972847);中国水产科学研究院中央级公益性科研院所基本科研业务费专项资金资助(2023TD44);中国水产科学研究院南海水产研究所中央级公益性科研院所基本科研业务费专项资金资助(2021SD05,2022XK02);广东省省级现代农业产业技术体系(2019KJ141)。

摘　　要：具有滤食习性的牡蛎富集了水体中包括病毒在内的大量病原体,是一个极具价值的病毒库。为了对牡蛎相关的病毒库进行深入研究,在前期对华南沿海多地采集的香港牡蛎(Crassostrea hongkongensis)进行病毒组测序,并对测序数据进行质控、组装及物种注释后,挑选其中5条被鉴定为微病毒科(Microviridae)的基因组序列进行多维度分析,包括宿主预测、开放阅读框预测、主要衣壳蛋白系统发育与三维结构预测、主要衣壳蛋白与外部支架蛋白的进化关联以及病毒丰度分析等。结果显示,5个病毒的宿主均为埃希氏菌属(Escherichia);其中病毒基因组序列HSd1-5344568聚类在Bullavirinae分支中,说明其为该亚科成员;其余4条未聚类到任何已知亚科中,应属于一个单独的未分类亚科;微病毒主要衣壳蛋白和外部支架蛋白的进化树之间的联系表明两个蛋白的进化规律不同。Oysters with filter feeding habits enrich a large number of pathogens including viruses in water,like a very valuable virus bank.To conduct an in-depth research on oyster-related viruses,we sequeced the Hong Kong China oyster(Crassostrea hongkongensis)collected along the South China coast.After the quality control,assembly and taxonomy annotation of the sequencing data,we selected five genomic sequences which were identified as Microviridae for multi-dimensional analyses,such as host pre-diction,open reading frame and gene function prediction,phylogeny and three-dimensional structure prediction of major capsid proteins,evolutionary association between major capsid proteins and external scaffold proteins,as well as virus abun-dance analysis.The results show that the hosts of the five viruses were all Escherichia;one of the virus genome sequences was clustered in Bullavirinae branch,which indicates that it is a member of the subfamily;the other four genome sequences were not clustered into any known subfamilies so they should belong to a single unclassified subfamily;the relationship between the evolu-tionary tree of the main capsid proteins and external scaffold proteins indicates that the evolution rules of the two proteins were different.

关 键 词：香港牡蛎 微病毒科 生物信息学分析 主要衣壳蛋白 外部支架蛋白 

分 类 号：S917.1[农业科学—水产科学]