加米霉素纳米乳化制剂的制备  

Preparation of Gamithromycin Nanoemulsion

作　　者：谢建瑛 苗耀天 亢永久 赵咏中 XIE Jian-ying;MIAO Yao-tian;KANG Yong-jiu;ZHAO Yong-zhong(National Agricultural Science and Technology Park Management Committee,Wuwei,Gansu 733000,China;Animal Husbandry and Veterinary Station,Wuwei,Gansu 733000,China;Wuwei Xinong Biotechnology Co.,Ltd,Wuwei,Gansu 733000,China)

机构地区：[1]甘肃武威国家农业科技园区管委会,甘肃武威733000 [2]武威市畜牧兽医总站,甘肃武威733000 [3]武威喜农生物科技有限责任公司,甘肃武威733000

出　　处：《动物医学进展》2023年第10期75-79,共5页Progress In Veterinary Medicine

基　　金：2022年度甘肃省科技计划项目-科技型中小企业技术创新基金项目(22CX3GH021)。

摘　　要：为了制备加米霉素纳米乳,并对其理化性质进行评价。充分考虑纳米乳的药效及稳定性,筛选合适的油相、表面活性剂,并利用伪三元相图筛选最佳配方制备纳米乳。用透射电镜观察加米霉素纳米乳的形态,用激光粒度分析仪测定其粒径分布范围、多分散系数等,通过留样观察测定其稳定性。结果表明,加米霉素纳米乳最佳配方中各成分比例(质量分数)为:蔗糖脂肪酸酯32.0%,加米霉素3.0%,IPM8.0%,无水乙醇4.0%,水53.0%。制备出的加米霉素纳米乳呈规则的圆球形,平均粒径13.7 nm,外观澄清透明,稳定性试验证实其稳定性良好,动物安全性试验证明在规定用量使用前提下其安全性良好。表明加米霉素纳米乳制备工艺可操作性强,质量稳定,使用安全,有较好的应用潜力。The purpose of this stduy was to establish Gamithromycin nanoemulsion formula and evaluate its quality.Considering the effect and stability of Gamithromycin nanoemulsion,the appropriate oil phase,surfactant were selected.Then the optimum formula was designed using Pseudo-ternary phase diagram.The configuration was measured by TEM,and the particle size,polydispersity and Zeta potential were measured by LPSA.The stability was tested by high speed centrifugation and optic observation.Results showed that the optimum formula of Gamithromycin nanoemulsion was composed of 32%(w/w)sucrose fatty acid esters,3.0%(w/w)gamithromycin,8.0%(w/w)IPM,4.0%(w/w)anhydrous ethanol,53.0%(w/w)distilled water.Electron microscopy showed that the Gamithromycin nanoemulsion was composed of small spherical drops with a mean diameter of 13.7 nm(25℃).Its stability was qualified,animal safety tests showed that it is safe to use at the prescribed dosage.The Gamithromycin nanoemulsion was successfully prepared with good stability,and the method of produce was simple and easy,there was a good utilization potentiality.

关 键 词：加米霉素 纳米乳化 新型制剂 

分 类 号：S853.74[农业科学—临床兽医学]