TSP-1对小鼠皮肤放创复合伤伤口愈合的影响  

Effect of TSP-1 on wound healing of skin combined radiation-wound injury in mice

作　　者：南莎 柳随义 冉永红 赵雅贞 郝玉徽 NAN Sha;LIU Sui-yi;RAN Yong-hong;ZHAO Ya-zhen;HAO Yu-hui(Institute of Combined Injury,College of Preventive Medicine,Army Medical University,Chongqing 400038,China)

机构地区：[1]陆军军医大学军事预防医学系复合伤研究所,重庆400038

出　　处：《局解手术学杂志》2023年第10期865-869,共5页Journal of Regional Anatomy and Operative Surgery

基　　金：国家自然科学基金(31024643)。

摘　　要：目的探讨TSP-1在小鼠皮肤放创复合伤(CRWI)伤口愈合过程中的作用。方法以TSP-1敲除(KO)小鼠为主要研究对象,以野生型(WT)小鼠作为对照,每组15只,比较WT小鼠与TSP-1KO小鼠皮肤CRWI的伤口愈合情况。取WT小鼠与TSP-1KO小鼠皮肤CRWI建模后第7天的伤口组织进行免疫组化染色以及免疫荧光染色,Western blot分析NF-кB蛋白表达水平,并使用RT-qPCR检测伤口部位炎症细胞因子IL-1β、IL-6和TNF-α的表达水平,以确定2组小鼠皮肤CRWI伤口部位的炎症反应状态及血管生成情况。结果与WT小鼠相比,从CRWI建模第3天开始TSP-1KO小鼠皮肤CRWI伤口愈合显著延迟(P<0.05)。CRWI建模后第7天TSP-1KO小鼠伤口部位的F4/80巨噬细胞浸润减少(P<0.05),NF-кB蛋白表达水平降低(P<0.01),炎症细胞因子IL-1β和IL-6的表达水平也相对降低(P<0.01),并且伤口部位CD31阳性细胞以及α-SMA阳性细胞数量减少(P<0.05)。结论敲除TSP-1会导致小鼠皮肤CRWI炎症反应减弱,血管生成受到抑制,伤口愈合延迟。Objective To investigate the effect of TSP-1 on wound healing process of skin combined radiation-wound injury(CRWI)in mice.Methods TSP-1 knockout(KO)mice were used as the main study subjects,and wild-type(WT)mice were used as the control,with 15 mice in each group.The wound healing of skin CRWI between WT mice with TSP-1KO mice was compared.The wound tissues of WT mice and TSP-1KO mice on the 7th day after skin CRWI modeling were taken for immunohistochemical staining and immunofluorescence staining,the NF-кB protein expression levels were analyzed by Western blot,and the expression levels of inflammatory cytokines IL-1β,IL-6 and TNF-αin the wound site were detected by RT-qPCR,so as to determine the inflammatory response and angiogenesis in the skin CRWI wound site of mice in the two groups.Results Compared with WT mice,skin CRWI wound healing was significantly delayed in TSP-1KO mice from the 3th day of CRWI modeling(P<0.05).On the 7th day after CRWI modeling,the infiltration of F4/80 macrophages in the wound site of TSP-1KO mice decreased(P<0.05),the NF-кB protein expression level decreased(P<0.01),the inflammatory cytokines IL-1βand IL-6 expression levels were also relatively reduced(P<0.01),and the number of CD31-positive cells as well asα-SMApositive cells in the wound site were decreased(P<0.05).Conclusion Knockout of TSP-1 can weaken the inflammatory response,inhibit angiogenesis and delay wound healing of skin CRWI in mice.

关 键 词：TSP-1 放创复合伤 伤口愈合 炎症反应 血管生成 

分 类 号：R146[医药卫生—公共卫生与预防医学]