肺动脉高压患儿血清miR-21、TWEAK表达及与术后危重评分的相关性  

Expression of serum miR-21 and TWEAK in children with pulmonary arterial hypertension and their correlation with postoperative critical illness score

作　　者：刘海红 杨春光 王凤东 崔树利 LIU Hai-hong;YANG Chun-guang;WANG Feng-dong;CUI Shu-li(Department of Neonatology,Chengde Maternal and Child Health Hospital,Chengde 067020,Hebei,China)

机构地区：[1]承德市妇幼保健院新生儿科,河北承德067020

出　　处：《广东医学》2023年第8期1008-1013,共6页Guangdong Medical Journal

基　　金：河北省医学科学研究课题计划(20200184)。

摘　　要：目的探讨肺动脉高压(PH)患儿血清微小RNA-21(miR-21)、肿瘤坏死因子样凋亡微弱诱导剂(TWEAK)表达及与术后危重评分的相关性。方法选取2019年5月至2022年1月PH患儿82例为观察组,另选取同期先天性心脏病(CHD)不伴PH的82例患儿为对照组。比较两组血清miR-21、TWEAK水平及术后危重评分,Pearson相关系数分析血清miR-21、TWEAK水平与术后危重评分的相关性,多重线性回归模型分析术后危重评分的影响因素。结果观察组血清miR-21水平高于对照组,血清TWEAK水平及术后危重评分低于对照组(P<0.05)。血清miR-21水平与术后危重评分呈负相关(r=-0.833,P<0.001),血清TWEAK水平与术后危重评分呈正相关(r=0.707,P<0.001)。经多元线性回归分析,血清miR-21及TWEAK水平对应的线性系数差异有统计学意义(P<0.05)。建立回归模型为:术后危重评分=4.021-2.214×miR-21+14.797×TWEAK,回归模型具有显著统计学意义(P<0.05),自变量可解释术后危重评分89.83%的变异量。结论PH患儿血清miR-21、TWEAK水平呈异常表达,且二者表达水平可用于预测患儿术后病情危重程度。Objective To investigate the expression of serum microRNA-21(miR-21)and tumor necrosis factor-like weak inducer of apoptosis(TWEAK)in children with pulmonary hypertension(PH)and their correlation with postoperative critical illness scores.Methods A total of 82 children with PH from May 2019 to January 2022 were selected as the observation group,and 82 children with congenital heart disease(CHD)without PH during the same period were selected as the control group.The serum miR-21 and TWEAK levels and postoperative critical illness scores were compared between the two groups.The Pearson correlation coefficient was used to analyze the correlation between serum miR-21 and TWEAK levels and postoperative critical illness score.Multiple linear regression models were used to analyze the influencing factors of postoperative critical care score.Results The serum miR-21 level in the observation group was significantly higher than that in the control group,and the serum TWEAK level and postoperative critical illness score were significantly lower than those in the control group(P<0.05).Serum miR-21 level was negatively correlated with postoperative critical score(r=-0.833,P<0.001);and serum TWEAK level was positively correlated with postoperative criticality score(r=0.707,P<0.05).After multiple linear regression analysis,the linear coefficients corresponding to serum miR-21 and TWEAK levels were significantly different(P<0.05).The regression model was established as follows,postoperative critical illness score=4.021-2.214×miR-21+14.797×TWEAK,and the regression model was statistically significant(P<0.05).Independent variables could explain 89.83%of the variance in postoperative critical score.Conclusion Serum levels of miR-21 and TWEAK are abnormally expressed in children with PH,and their expression levels can be used to predict the severity of postoperative illness in children.

关 键 词：肺动脉高压 MIR-21 TWEAK 术后危重评分 

分 类 号：R816.92[医药卫生—放射医学] R56[医药卫生—临床医学]