基于胃肠黏膜屏障探讨不同频率摩腹对脾虚证家兔的治疗效应研究  

Based on Gastrointestinal Mucosal Barrier to Explore The Therapeutic Effect of Different Frequency of Abdominal Rubbing on Rabbits with Spleen Deficiency

作　　者：许嘉英 王继红 黄志凯 任雪晗 张强 蔡伟蓝 XU Jiaying;WANG Jihong;HUANG Zhikai(Clinical College of Acu-puncture,Moxibustion and Rehabilitation Guangzhou University of Chinese Medicine,Guangzhou Guangdong)

机构地区：[1]广州中医药大学针灸康复临床医学院,广东广州510405

出　　处：《四川中医》2023年第6期62-68,共7页Journal of Sichuan of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(编号:81873395)。

摘　　要：目的:通过观察不同频率摩腹对脾虚家兔胃肠黏膜屏障的作用,探讨摩腹频率与治疗效应之间的关系。方法:选取普通成年新西兰兔72只,随机分成空白组K、模型组P、低频摩腹组A(频率101~150次/分)、高频摩腹组B(频率201~250次/分)、低频抑制剂组I1、高频抑制剂组I2。K组不予处理,其余5组予大黄芒硝番泻叶合剂灌胃造模。造模结束后,采用机械臂分别对A组、B组、I1组、I2组进行10天不同频率摩腹干预,其中I1组及I2组在手法干预前均予腹腔注射ML-9抑制剂(2mg·kg^(-1))。10天后进行胃肠黏膜标本采集,以各组标本的黏膜上皮细胞数、黏膜上皮长度和HE染色病理切片作为观察指标。通过比较光镜下各组家兔胃、肠黏膜病理组织学改变、黏膜上皮细胞数和黏膜上皮长度,分析摩腹的治疗效应。结果:与空白组K相比,模型组P的胃、肠黏膜上皮细胞数及黏膜上皮长度显著减少(P<0.05),病理切片提示大量炎性细胞浸润。与模型组P相比,A组及I1组脾虚家兔的胃、肠黏膜上皮细胞数及黏膜上皮长度明显增加,病理切片提示黏膜上皮细胞结构规则。而B组及I2组脾虚家兔的胃、肠黏膜上皮细胞数及黏膜上皮长度虽有一定程度的增加,但与模型组P的差异不显著(P>0.05),且病理切片提示仍有炎性细胞浸润,黏膜上皮细胞结构不规则。I1组与K组、P组比较均有显著差异(P<0.05)。结论:(1)摩腹频率介于101~150次/分有助于胃肠黏膜上皮细胞修复增生,对脾虚型胃肠黏膜屏障疾病产生修复作用,而频率介于201~250次/分的摩腹对脾虚型胃肠黏膜屏障疾病则无明显治疗作用。(2)在ML-9抑制剂抑制肌球蛋白轻链激酶(Myosin light-chain kinase, MLCK)表达的情况下,低频率摩腹产生的治疗效应受影响,说明MLCK通路是摩腹产生疗效的信号通路之一。(3)在ML-9抑制剂抑制MLCK表达的情况下,低频率摩腹仍能通过其他靶点或信号通�Objective To explore the relationship between the frequency and therapeutic effect of abdominal rubbing by ob-serving the effect of abdominal rubbing with different frequencies on gastrointestinal mucosal barrier in rabbits with spleen defi-ciency.Methods 72normal adult New Zealand rabbits were randomly divided into blank group K,model group P,low-frequency group A(The frequency of rubbing at 101~150times/min),high-frequency group B(The frequency of rubbing at 201~25Otimes/min),low-frequency inhibitor group Iland high-frequency inhibitor group I2.Except Group K,the other groups were modeled with the mixture includes rhubarb,mirabilite and senna,gavage administration.After the modeling,Groups A,B,Iland I2were subjected to a 10-day abdominal rubbing intervention by means of mechanical arms.Before each intervention,Group Iland I2received an intraperitoneal injection of ML-9(2mg·kg^(-1)).Gastrointestinal mucosa samples were collected 10days later.The number of gastric and intestinal epithelial cells,the length of mucosal epithelium and HE staining pathological sections of each group were used as observation indicators.The therapeutic effect of abdominal rubbing was analyzed by comparing the histopathological changes,the number of gastric and intestinal epithelial cells and the length of mucosal epithelium of rabbits in each group under light microscope.Results Compared with blank group K,the number of gastric and intestinal epithelial cells and the length of mucosal epithelium inmodel group P were significantly reduced(P<0.05)and pathological sections showed a large number of inflammatory cell infiltration.Compared with model group P,the number of gastric and intestinal epithelial cells and the length of mucosal epithelium in Group A and Ilwere significantly increased and pathological sections showed that the structure of mucosal epithelial cells was regular.Although the number of gastric and intestinal epithelial cells and the length of mucosal epithelium in Group B and I2increased to a certain extent,there was no

关 键 词：摩腹 手法频率 治疗效应 胃肠黏膜屏障 脾虚 

分 类 号：R244.1[医药卫生—针灸推拿学]