靶向MPO的次氯酸荧光探针用于细胞成像  

Hypochloric acid fluorescence probe targeting MPO for cell imaging

作　　者：徐辉军 王玉婷 秦亚娟[1] 厉廷有[1] XU Huijun;WANG Yuting;QIN Yajuan;LI Tingyou(School of Pharmacy,Nanjing Medical University,Nanjing 211166,China)

机构地区：[1]南京医科大学药学院,江苏南京211166

出　　处：《南京医科大学学报（自然科学版）》2023年第10期1342-1349,共8页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家自然科学基金(81573280)。

摘　　要：目的:合成靶向髓过氧化物酶(myeloperoxidase,MPO)的次氯酸(hypochloric acid,HClO)荧光探针并评估其HClO示踪活性。方法:以硅罗丹明为荧光团合成SiRho-GABA-KYC,紫外分光光度计和荧光分光光度计检测其紫外光谱和荧光光谱以及pH稳定性和选择性,CCK-8法检测细胞毒性,用共聚焦显微镜进行细胞荧光成像。结果:合成了SiRho-GABA-KYC,对HClO响应高,选择性好,细胞毒性小,可示踪HeLa细胞中HClO浓度的变化。结论:探针SiRho-GABA-KYC可用于检测HClO浓度进而反映MPO催化活性。Objective:The current study aims to synthesize hypochloric acid(HClO)fluorescent probes targeting myeloperoxidase(MPO)and evaluate its HClO tracing activity.Methods:Si⁃rhodamine was used as the fluorophore to synthesize SiRho⁃GABA⁃KYC.The UV spectrum,fluorescence spectrum,and pH stability and selectivity were detected with UV spectrophotometer and fluorescence spectrophotometer.Cytotoxicity was detected with CCK⁃8 method,and cell fluorescence imaging was performed by confocal microscopy.Results:SiRho⁃GABA⁃KYC was synthesized,with a high response to HClO,a good selectivity,and a low cytotoxicity,which can trace changes in HClO concentration in HeLa cells.Conclusion:The probe SiRho⁃GABA⁃KYC can be used to detect HClO concentration,which in turn reflects MPO catalytic activity.

关 键 词：髓过氧化物酶 次氯酸 荧光探针 

分 类 号：R446[医药卫生—诊断学]