斑马鱼肌肉Smyd1a蛋白生物信息学分析  

Bioinformatics Analysis of Zebrafish Muscle Development with Smyd1a

作　　者：杨明勇 麦晓雯 张在宝 刘慧娟 李红敬 无 YANG Mingyong;MAI Xiaowen;ZHANG Zaibao;LIU Huijuan;LI Hongjing;无(College of Physical Education,Xinyang Normal University,Xinyang 464000,China;College of Life Sciences,Xinyang Normal University,Xinyang 464000,China;College of Science,Qiongtai Normal University,Haikou 571127,China;Tropical Biodiversity and bioresource Utilization Laboratory,Qiongtai Normal University,Haikou 571127,China)

机构地区：[1]信阳师范大学体育学院,河南信阳464000 [2]信阳师范大学生命科学学院,河南信阳464000 [3]琼台师范学院理学院,海南海口571127 [4]琼台师范学院热带生物多样性与资源利用实验室,海南海口571127

出　　处：《信阳师范学院学报（自然科学版）》2023年第4期575-579,共5页Journal of Xinyang Normal University(Natural Science Edition)

基　　金：国家自然科学基金项目(32170351);河南省科技攻关项目(202102110110)。

摘　　要：通过使用美国国立生物技术信息中心(National Center for Biotechnology Information,NCBI)数据库检索出斑马鱼Smyd1a蛋白的氨基酸序列信息,对其进行生理生化分析,并通过NBCI寻找同源性高的不同种属的序列构建系统发育树。结果显示:Smyd1a蛋白的氨基酸数量是489个,分子量是56376.54 Da,理论等电点是6.27;该蛋白中,谷氨酸(Glu)含量最高,为8.4%;其次是亮氨酸(Leu)和丙氨酸(Ala),分别为8.0%和7.0%。Smyd1a是一个亲水蛋白质,但不是一个分泌蛋白,不存在跨膜螺旋区域,存在蛋白质的无规则卷曲等构象,Smyd1a属于SET家族。The amino acidebrafish Smyd1a protein was retrieved from the National Center for Biotechnology Information(NCBI)database,and subsequent physiological and biochemical analyses were conducted.Phylogenetic trees were constructed by searching for highly homologous sequences from different species using NCBI.The results revealed that Smyd1a protein consists of 489 amino acids with a molecular weight of 56376.54 Da and a theoretical isoelectric point of 6.27.Glutamic acid(Glu)exhibited the highest content at 8.4%,followed by leucine(Leu)and alanine(Ala)at 8.0%and 7.0%,respectively.Smyd1a is characterized as a hydrophilic protein without secretion lacking transmembrane helical regions while displaying irregular folding patterns within its structure.Furthermore,Smyd1a belongs to the SET family.

关 键 词：斑马鱼 Smyd1a 氨基酸序列 亲水性蛋白质 分泌蛋白 系统发育树 

分 类 号：Q445[生物学—生理学]