两个南方红豆杉天然居群的交配系统分析  

Mating system analyses of two natural populations of Taxus wallichiana var.mairei

作　　者：罗芊芊 李峰卿[3] 肖德卿 邓章文 王建华 周志春[2] LUO Qianqian;LI Fengqing;XIAO Deqing;DENG Zhangwen;WANG Jianhua;ZHOU Zhichun(Forestry Research Institute of Fuzhou,Fuzhou 344000,China;Research Institute of Subtropical Forestry,Chinese Academy of Forestry,Hangzhou 311400,China;Experimental Center of Subtropical Forestry,Chinese Academy of Forestry,Fenyi 336600,China)

机构地区：[1]抚州市林业科学研究所,江西抚州344000 [2]中国林业科学研究院亚热带林业研究所,浙江杭州311400 [3]中国林业科学研究院亚热带林业实验中心,江西分宜336600

出　　处：《南京林业大学学报（自然科学版）》2023年第5期80-86,共7页Journal of Nanjing Forestry University：Natural Sciences Edition

基　　金：百山祖国家公园科学研究项目(2021ZDLY02);中国林业科学研究院中央级公益性科研院所基本科研业务费专项资金项目(CAFYBB2018ZB010);抚州市科技计划项目(2018Tc06)。

摘　　要：【目的】研究南方红豆杉(Taxus wallichiana var.mairei)天然居群的交配系统,揭示浙江龙泉和江西分宜天然居群的交配机制以及亲代间的遗传多样性差异,为南方红豆杉的科学保育和遗传资源有效利用提供理论参考。【方法】以浙江龙泉和江西分宜天然居群亲代及其子代为研究对象,运用12对SSR引物对71个亲代及8个采种母株的104个子代进行分析。【结果】参试的浙江龙泉和江西分宜居群亲代的等位基因数(N_(a))皆为7,2个参试居群的有效等位基因数(N_(e))分别为4.831和3.552,浙江龙泉居群较江西分宜居群高出1.279个有效等位基因。2个参试居群的观测杂合度(H_(o))均小于期望杂合度(H_(e)),且固定指数(F)大于0。浙江龙泉居群的等位基因丰富度和私有等位基因丰富度(A_(R)=4.195,P_(A)=1.832)皆大于江西分宜居群(A_(R)=3.576,P_(A)=1.647)。2个参试居群的遗传多样性略有差异,但仍具有较高的遗传多样性。多位点交配系统分析(MLTR)结果表明,浙江龙泉和江西分宜居群中,南方红豆杉的异交率较高(t_(m)=1.200)。单位点和多位点父本相关性的差值[r_(p(s))-r_(p(m))]为0.095,亲代居群内存在近交现象,但近交水平较低(C_(t_(m)−t_(s))=0.268)。有效花粉供体(N_(ep)=1.5)数目较少,表明南方红豆杉自然交配时亲本(父本)来源非常有限。【结论】南方红豆杉属于高度异交树种,2个参试居群的异交率差异不明显且遗传多样性皆较高,居群内存在近交现象,但近交水平较低。【Objective】The objective of this study is to examine the mating system of Taxus wallichiana var.mairei in natural populations located in Longquan City from Zhejiang Province and Fenyi City from Jiangxi Province.Furthermore,the study aims to elucidate the influence of mating mechanisms and variances in parental genetic diversity,to provide a theoretical basis for the scientific conservation and efficient utilization of the genetic resources of T.wallichiana var.mairei.【Method】A total of 71 parental plants and 104 progenies stemming from eight mother plants were meticulously analyzed using 12 pairs of the SSR primers,all of which were sourced from natural populations located in Longquan of Zhejiang Province(ZJLQ)and Fenyi of Jiangxi Province(JXFY).With the aid of GenAlEx 6.5,a number of genetic diversity measures such as the observed alleles and effective alleles,as well as observed heterozygosity,expected heterozygosity,Shannon index and fixed index were meticulously calculated.Furthermore,the team also utilized HP-Rare 1.1 to calculate allele richness and private allele richness,while FSTAT 2.9.3 was utilized to test the genetic diversity parameters for any potential differences among parents within each population through 1000 simulations,along with the calculation of the inbreeding coefficient of the parental population.Lastly,we used MLTR 3.4 to estimate thoroughly the genetic parameters of T.wallichiana var.mairei’s mating system,which included single-locus population outcrossing rate,multilocus population outcrossing rate,number of effective pollen donors,multilocus correlation of paternity,singlelocus correlation of paternity,difference between the paternal correlation of single-locus and multilocus.【Result】The average allele of the parental generation from Longquan and Fenyi populations was 7.Meanwhile,the effective allele number(N_(e)),Shannon index(I),allele richness(A_(R))and private allele richness(P_(A))of the two parental populations were 4.192,1.508,3.886 and 1.740,respectively.In comp

关 键 词：南方红豆杉 天然居群 交配系统 亲代 子代 微卫星DNA 

分 类 号：S722[农业科学—林木遗传育种]