“四白”中药复方醇提液的安全性及护肤作用研究  

Research of Biosafety and Skin-Care Efficacy on Alcoholic Extract of Chinese Herbal Compound“Four Whites”

作　　者：唐子溦 王艳 叶小花 谢锌仪 罗玺 时军 赵力民 赵平 TANG Ziwei;WANG Yan;YE Xiaohua;XIE Xinyi;LUO Xi;SHI Jun;ZHAO Limin;ZHAO Ping(School of Chemistry and Chemical Engineering,Guangdong Pharmaceutical University,Zhongshan 528458 Guangdong,China;School of Traditional Chinese Medicine,Guangdong Pharmaceutical University,Guangzhou 510006 Guangdong,China)

机构地区：[1]广东药科大学医药化工学院,广东中山528458 [2]广东药科大学中药学院,广东广州510006

出　　处：《中药新药与临床药理》2023年第9期1219-1226,共8页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：广东省普通高校重点领域专项(2021ZDZX4019);广东省药品监督管理局科技创新项目(2021YDZ05);中山市社会公益与基础研究专项(2020B2023)。

摘　　要：目的研究白僵蚕、白蔹、白术及白芍(“四白”)中药复方醇提液的安全性及护肤(美白、抗氧化、抗炎)功效。方法采用红细胞溶血实验及MTT法检测细胞存活率,评估“四白”中药复方醇提液的眼刺激性及细胞安全性;通过羟自由基、1,1-二苯基-2-三硝基苯肼(DPPH)自由基清除实验、铁离子(Fe^(3+))还原实验和抗过氧化氢(H2O2)氧化损伤实验评估“四白”中药复方醇提液的抗氧化能力;通过酪氨酸酶活性抑制实验评估“四白”中药复方醇提液的美白功效;通过透明质酸酶活性抑制实验和采用ELISA法检测脂多糖(LPS)诱导RAW264.7细胞一氧化氮(NO)水平,评价“四白”中药复方醇提液的抗炎功效。结果(1)与0.1%十二烷基硫酸钠(SDS)组比较,30 g·L^(-1)“四白”中药复方醇提液组的红细胞溶血率显著降低(P<0.01)。与空白组比较,“四白”中药复方醇提液(5、10、15、20、25、30 g·L^(-1))不同浓度组的HaCaT、B16及RAW264.7细胞活力均显著降低(P<0.05,P<0.01);烟酰胺对B16细胞的IC50值为(26.3±1.3)g·L^(-1),低于“四白”中药复方醇提液对B16细胞的IC50值[(33.8±2.7)g·L^(-1)]。(2)与空白组比较,“四白”中药复方醇提液(2、4、8、16、32 g·L^(-1))的羟自由基清除率显著升高(P<0.01);“四白”中药复方醇提液(0.25~4 g·L^(-1))的DPPH自由基清除率显著升高(P<0.01);“四白”中药复方醇提液(5、10、15、20、25、30 g·L^(-1))对Fe^(3+)的还原力显著增强(P<0.01);与空白组比较,模型组HaCaT细胞活力显著降低(P<0.01),而“四白”中药复方醇提液(5、10、15、20、25、30 g·L^(-1))预保护后,HaCaT细胞活力有上升趋势,但差异无统计学意义(P>0.05)。(3)随着“四白”中药复方醇提液浓度的升高,酪氨酸酶活性抑制率逐渐升高,在30 g·L^(-1)浓度时,活性抑制率达到92.1%;与空白组比较,“四白”中药复方醇提液(15、20、25、30 g·L^(-1))对B16细胞的酪氨酸酶活性�Objective To study the biosafety and skincare(whitening,anti-oxidatidant,anti-inflammatory)effects of the alcoholic extracts of the Chinese herbal compound Bombyx Batryticatus,Ampelopsis Radix,Atractylodis Macrocephalae Rhizoma and Paeoniae Radix Alba(namely,the"four whites")were investigated.MethodsThe cell viability and cell safety of alcoholic extract of"four whites"were assessed by red blood cell haemolysis assay and MTT assay;the antioxidant effect of alcoholic extract of"four whites"was assessed by hydroxyl radical,1,1-diphenyl-2-trinitrophenyl hydrazine(DPPH)radical scavenging assay,iron ion(Fe^(3+))reduction assay and antihydrogen peroxide(H_(2)O_(2))oxidative damage assay.The whitening efficacy of alcoholic extract of"four whites"was evaluated by tyrosinase activity inhibition assay;the anti-inflammatory efficacy of alcoholic extract of"four whites"was evaluated by hyaluronidase activity inhibition assay and ELISA assay to detect the NO level of LPS-induced RAW264.7 cells,and the anti-inflammatory efficacy of the alcoholic extract of"four whites"was evaluated.Results(1)Compared with the 0.1%SDS group,the haemolysis rate of erythrocytes in the 30 g·L^(-1)alcoholic extract of"four whites"group was significantly decreased(P<0.01).Compared with the blank group,the viability of HaCaT,B16 and RAW264.7 cells was significantly decreased(P<0.05,P<0.01)in the groups with different concentrations of alcoholic extract of"four whites"(5,10,15,20,25 and 30 g·L^(-1));the IC_(50)value of nicotinamide on B16 cells was(26.3±1.3)g·L^(-1),which was lower than that of alcoholic extract of"four whites"on B16 cells(33.8±2.7)g·L^(-1).(2)Compared with the blank group,the hydroxyl radical scavenging rate of alcoholic extract of"four whites"(2,4,8,16 and 32 g·L^(-1))was significantly increased(P<0.01);the DPPH radical scavenging rate of alcoholic extract of"four whites"(0.25-4 g·L^(-1))was significantly increased(P<0.01);the scavenging rate of DPPH radicals was significantly increased in the alcoholic extract of"four white

关 键 词：白僵蚕 白蔹 白术 白芍 安全性 护肤 抗氧化 美白 抗炎 

分 类 号：R285.5[医药卫生—中药学]