基于血清非靶向代谢组学研究肝喜片抗肿瘤的作用机制  

Antitumor mechanism of Ganxi tablets:A study based on serum non-targeted metabolomics

作　　者：陈玲珑 赵荻 彭艳梅[1,2] CHEN Linglong;ZHAO Di;PENG Yanmei(Hunan University of Chinese Medicine,Changsha 410208,Hunan,China;Hunan Academy of Chinese Medicine,Changsha 410006,Hunan,China)

机构地区：[1]湖南中医药大学,湖南长沙410208 [2]湖南省中医药研究院,湖南长沙410006

出　　处：《湖南中医杂志》2023年第9期147-153,共7页Hunan Journal of Traditional Chinese Medicine

基　　金：湖南省长沙市科技局项目(kq2004047)。

摘　　要：目的:利用超高效液相-质谱联用技术(UPLC-MS/MS)研究肝喜片在大鼠血清中的代谢调节机制。方法:将30只雄性SD大鼠随机分为对照组、肝喜片低剂量组(0.81 g/kg)、肝喜片高剂量组(4.05 g/kg),每组各10只,连续给药7 d后腹主动脉采血,采用UPLC-MS/MS检测血清代谢物变化情况。结果:对照组和给药组的代谢轮廓具有明显变化,通过设定VIP值>1、P<0.05阈值筛选差异代谢物。肝喜片低剂量组与对照组之间差异代谢物主要有40种,肝喜片高剂量组与对照组之间差异代谢物主要有77种,3组之间共有的差异代谢物有26个,其中涉及到的代谢途径主要包括戊糖和葡萄糖醛酸的相互转化、色氨酸代谢和类固醇激素生物合成。结论:肝喜片抗肿瘤的作用机制可能与戊糖和葡萄糖醛酸的相互转化、色氨酸代谢和类固醇激素生物合成等代谢途径调控有关。利用代谢组学方法研究差异代谢物变化可以为抗肿瘤提供治疗靶点。Objective:To investigate the metabolic regulation mechanism of Ganxi tablets in rat serum by ultra-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).Methods:A total of 30 male Sprague-Dawley rats were randomly divided into control group,low-dose Ganxi tablets group(0.81 g/kg),and high-dose Ganxi tablets group(4.05 g/kg),with 10 rats in each group.Blood samples were collected from the abdominal aorta after administration for 7 consecutive days,and UPLC-MS/MS was used to measure the changes in serum metabolites.Results:The control group and the administration groups had significant changes in the metabolic profile,and differentially expressed metabolites were obtained based on VIP value>1 and P<0.05.There were mainly 40 differentially expressed metabolites between the low-dose Ganxi tablets group and the control group and 77 differentially expressed metabolites between the high-dose Ganxi tablets group and the control group,with 26 differentially expressed metabolites between the three groups,which involved the metabolic pathways such as the mutu-al transformation between pentose and glucuronic acid,the metabolism of tryptophan,and the biosynthesis of steroid hormone.Conclusion:The antitumor mechanism of Ganxi tablets might be associated with the metabolic pathways such as the mutual transformation between pentose and glucuronic acid,the metabolism of tryptophan,and the biosynthesis of steroid hormone.The research on the changes in differentially expressed metabolites based on metabolomics can provide therapeutic targets for antitumor treatment.

关 键 词：代谢组学 肝喜片 抗肿瘤 作用机制 

分 类 号：R285.5[医药卫生—中药学]