造纸废水活性污泥的微生物群落结构及多样性分析  

Microbial Community Structure and Diversity Analysis of Activated Sludge from Papermaking Wastewater

作　　者：王硕[1] 李长波[1] 许洪祝 赵国峥[1] 梁慧 李东彧 WANG Shuo;LI Chang-bo;XU Hong-zhu;ZHAO Guo-zheng;LIANG Hui;LI Dong-yu(Schl.of Environ.&Safety Engin.,Liaoning Petrochemical.Uni.,Fushun 113001)

机构地区：[1]辽宁石油化工大学环境与安全工程学院,辽宁抚顺113001

出　　处：《微生物学杂志》2023年第3期19-29,共11页Journal of Microbiology

基　　金：国家水体污染控制与治理科技重大专项(2012ZX07202-002);辽宁省教育厅重点攻关项目(L2020003);辽宁省教育厅项目(L2020020)。

摘　　要：为探究造纸废水活性污泥中微生物群落结构多样性以及对造纸废水处理效果的影响,利用Illumina MiSeq高通量测序方法,分析在处理造纸废水过程中,同一运行阶段两个并联氧化沟内活性污泥的微生物群落与多样性组成。结果表明,系统中处理造纸废水的活性污泥在同一废水条件下微生物群落结构总体稳定,优势细菌为绿弯菌门(Chloroflexi)、拟杆菌门(Bacteroidota)、变形菌门(Proteobacteria)、Myxococcota、放线菌门(Actinobacteria)、厚壁菌门(Firmicutes)等。最重要的优势细菌类群为Chloroflexi,相对丰度占比为47.67%~48.22%,远远高于其他废水中Chloroflexi的占比,其中厌氧绳菌纲(Anaerolineae)是其主要成员,占比84.39%~88.34%,可针对性地去除造纸废水中的污染物。造纸废水活性污泥样品中存在大量特殊功能菌群,其在废水中污染物尤其是木质素的去除中发挥着重要作用。In order to explore the diversity of microbial community structure in activated sludge of papermaking wastewater and its impact on the treatment effect of papermaking wastewater,the microbial community and diversity composition of activated sludge in two parallel oxidation ditches in the same operation stage under the condition of papermaking wastewater were analyzed by Illumina miseq high-throughput sequencing method.The results showed that the microbial community structure of activated sludge for papermaking wastewater treatment in the system was generally stable under the same wastewater conditions,and the dominant bacteria were Chloroflexi,Bacteroidota,Proteobacteria,Myxococcota,Actinobacteria,Firmicutes etc;The most important dominant bacterial group is Chloroflexi,with proportion abundance of 47.67%-48.22%,much higher than proportion of Chloroflexi in other wastewater.Among them Anaerolineae is its main member,with proportion of 84.39%-88.34%,which can remove targeted pollutants in papermaking wastewater.There are a large number of special functional bacteria in activated sludge samples of papermaking wastewater,which play an important role in the removal of pollutants,especially lignin.

关 键 词：细菌群落结构 造纸废水 活性污泥 高通量测序 污泥膨胀 丝状菌 

分 类 号：Q938.15[生物学—微生物学]