灵芝多糖肽GL-PPSQ_(2)的热分析研究  

Thermal analysis of Ganoderma lucidum polysaccharide peptide GL-PPSQ_(2)

作　　者：罗虹建[1,2] 王赛贞 王联福[1] 林树钱 鲁国东[1,3] 林占熺[1] 林冬梅[1] LUO Hong-jiann;WANG Sai-zhen;WANG Lian-fu;LIN Shu-qian;LU Guo-dong;LIN Zhan-xi;LIN Dong-mei(National Engineering Research Center of JUNCAO Technology Fujian Agriculture and Forestry University,Fuzhou 350002,China;College of Life science,Fujian Agriculture and Forestry University,Fuzhou 350002,China;College of Plant Protection,Fujian Agriculture and Forestry University,Fuzhou 350002,China)

机构地区：[1]福建农林大学国家菌草工程技术研究中心,福州350002 [2]福建农林大学生命科学学院,福州350002 [3]福建农林大学植物保护学院,福州350002

出　　处：《药物分析杂志》2023年第9期1468-1475,共8页Chinese Journal of Pharmaceutical Analysis

基　　金：中央引导地方科技发展专项(2021L3008);福建省重大专项“菌草种质创新及其产业化利用关键技术研究与应用”(2021NZ0101);学科交叉融合推动菌草科学及产业高质量发展(XKJC71202103A)。

摘　　要：目的:研究灵芝多糖肽(GL-PPSQ_(2))的热解物理化学特性和热稳定性。方法:采用热重(TG)和红外(IR)、质谱(MS)联用,在30~800℃升温范围为内对GL-PPSQ_(2)进行热重(TG)和微分热重(DTG)分析;采用差示扫描量热(DSC)法,升温范围为-50~500℃,流量为50 mL·min^(-1),升温速率为10℃·min^(-1),分别在氮气和空气气氛下测定样品的变性温度和热流变化。结果:GL-PPSQ_(2)的主要热解温度为180~450℃,在310℃(氮气)和265℃(空气)出现最大失重尖峰,失重过程在600℃左右结束,800℃时热解残留量分别为14.78%(氮气)和1.65%(空气)。TG-IR和TG-MS结果表明,热解产物析出量在320℃时达到最大峰值,小分子气体产物主要有H2O和CO_(2)。对热处理样品残留物进行红外光谱分析主要含有多糖、碳和硫酸盐。DSC分析可知,GL-PPSQ_(2)在200℃下吸热峰为失去吸附水或溶剂挥发,热解过程发生在200~500℃,氧化分解放热量(空气)比热解放热量大(氮气)。结论:研究灵芝多糖肽的热解特性和热稳定性,为其研制标准物质和开发药物提供参考基础。Objective: To study the pyrolytic physicochemical properties and thermal stability of Ganoderma lucidum polysaccharide peptide(GL-PPSQ_(2)). Methods: The thermogravimetric(TG) and differential thermogravimetric(DTG) analysis of GL-PPSQ_(2) were carried out in the temperature range of 30-800 ℃ using TG combined infrared(IR) and mass spectrometry(MS). The denaturation temperature and heat flow of GL-PPSQ_(2) was measured by differential scanning calorimetry(DSC) in the temperature range of-50-500 ℃ with a flow rate of 50 mL·min^(-1) and heating rate of 10 ℃·min^(-1) under nitrogen(N_2) and air atmosphere, respectively. Results: The main pyrolysis temperature of GL-PPSQ_(2) ranged in 180-450 ℃, and there were a weightlessness peak at 310 ℃(N_2) and 265 ℃(air). The weightlessness process ended around 600 ℃, and the residual amount of pyrolysis were 14.78%(N_2) and 1.65%(air) at 800 ℃, respectively. TG-IR and TG-MS results indicated that the pyrolysis product of GL-PPSQ_(2) reached the highest at 320 ℃. The small molecule gas products mainly included H_2O and CO_(2). The residue of heat-treated GL-PPSQ_(2) mainly contained polysaccharides, carbon and sulfate by infrared spectroscopy analysis. DSC analysis showed that the endothermic peak of GL-PPSQ_(2) below 200 ℃ was the loss of adsorbed water or solvent volatilization. The pyrolysis process occurred at 200 to 500 ℃, and the heat of oxidative decomposition(air) was greater than that of thermal degradation(N_2). Conclusion: Studying the pyrolytic properties and thermal stability of GL-PPSQ_(2) may provide a reference basis for the development of reference materials and drugs.

关 键 词：灵芝 多糖肽 热分析 热解特性 热重 热重-红外联用 热重-质谱联用 差示扫描量热法 

分 类 号：R917[医药卫生—药物分析学]