中国山杨群体CDS遗传多样性格局与谱系地理分析  

Population CDS Genetic Diversity and Phylogeography of Populus davidiana

作　　者：赵丹丹 张建国[1] 惠楠 陈为涛 王兆山[1] ZHAO Dan-dan;ZHANG Jian-guo;HUI Nan;CHEN Wei-tao;WANG Zhao-shan(Research Institute of Forestry,Chinese Academy of Forestry,Key Laboratory of Silviculture of the State Forestry Administration,Beijing 100091,China;Junan County Natural Conservation Area Management and Service Center,Shandong Junan 276600)

机构地区：[1]中国林业科学研究院林业研究所,国家林业局森林培育重点实验室,北京100091 [2]莒南县自然保护地管理服务中心,山东莒南276600

出　　处：《林业科学研究》2023年第5期12-20,共9页Forest Research

基　　金：中国林业科学研究院林业研究所基本科研业务费项目(ZDRIF201902);国家自然科学基金项目(31770702)。

摘　　要：[目的]基于REF6与BRR2a基因,探讨中国山杨不同群体遗传多样性分布格局、迁移路线,以及群体遗传变异与环境变化的关系。[方法]获取REF6与BRR2a基因的CDS序列,计算不同CDS序列的分子多样性指数,构建单倍型网络图以及系统发育树。[结果]在西南群体中,REF6基因和BRR2a基因3种中性检验的平均值均为负值,核苷酸多态性均低于北方和中部群体。中国山杨群体系统发育树与单倍型网络图结果显示外类群首先与中国山杨北方群体聚集在一起,西南群体往往聚集在末端,且两种基因的单倍型在北方与中部多样性较为丰富,西南群体中单倍型种类相对单一。[结论]中国山杨从北方迁移到南方,为了适应云贵高原独特的气候环境,位于西南地区的群体经历了强烈的自然选择,发生了局部适应。[Objective]Based on the REF6 and BRR2a genes,this study aimed to explore the distribution of the genetic diversity,the route of migration and the relationship between population genetic variation and environmental change of different populations of Populus davidiana.[Method]After obtaining the CDS sequences of REF6 and BRR2a genes,we calculated the molecular diversity index,constructed the haplotype network maps and the phylogenetic trees of the different CDS sequences.[Result]In the southwestern population,the mean of the three neutral tests for the REF6 and the BRR2a gene were negative,and the nucleotide polymorphisms were lower than those in both the northern and central populations.The result of phylogenetic tree and haplotype network maps showed that the outgroup first clustered with the northern population of P.davidiana.The southwest population tended to gather at the end,and the haplotypes of the two genes were abundant in the north and the central population.The haplotypes in the southwestern population were relatively single.[Conclusion]P.davidiana migrates from the north to the south.In order to adapt to the climate of Yunnan-Guizhou Plateau,population located in the southwest region experiences strong natural selection,and local adaptation occurs in the southwest populations.

关 键 词：中国山杨 REF6 BRR2a 遗传多样性 迁移路线 

分 类 号：Q941[生物学—植物学]