甜瓜双单倍体组培生根过程中内源激素、多胺及相关氧化酶活性的变化  

Dynamic changes in polyamines,endogenous hormones and oxidase activities during rooting of double haploid muskmelon in vitro plantlets

作　　者：赵卫星[1] 高宁宁[1] 康利允[1] 程志强 梁慎[1] 常高正[1] 李海伦 王慧颖 徐小利[1] 李晓慧[1] ZHAO Weixing;GAO Ningning;KANG Liyun;CHENG Zhiqiang;LIANG Shen;CHANG Gaozheng;LI Hailun;WANG Huiying;XU Xiaoli;LI Xiaohui(Institute of Horticulture,Henan Academy of Agricultural Sciences,Zhengzhou 450002,Henan,China;Kaifeng Agricultural and Forestry Research Institute,Kaifeng 475004,Henan,China)

机构地区：[1]河南省农业科学院园艺研究所,郑州450002 [2]开封市农林科学研究院,河南开封475004

出　　处：《果树学报》2023年第10期2195-2203,共9页Journal of Fruit Science

基　　金：农业农村部财政部:国家西甜瓜产业技术体系建设项目(CARS-25);河南省西甜瓜产业技术体系建设项目(HARS-22-10-G1);河南省科技攻关项目(222102110208);河南农业科学院自主创新项目(2023ZC033)。

摘　　要：【目的】探究甜瓜双单倍体组培苗内源激素、多胺类物质和关联酶对不定根诱导分化和伸长响应的生理机制。【方法】以未受精子房离体培养获得的双单倍体芽为试材,在生根培养基上诱导培养,探讨和分析其生根过程中内源激素、多胺类物质含量以及相关酶活性的变化规律。【结果】生根诱导过程中吲哚乙酸(IAA)、脱落酸(ABA)含量呈现“下降-升高”的趋势,分别在第7天和第14天达到最低值;赤霉素(GA_(3))含量先升高后下降,在第7天时达到最大值;玉米素核苷(ZR)含量呈现“下降-升高-下降”的趋势,在第21天达到最大值;IAA/ABA和过氧化物酶(POD)活性逐渐升高,IAA/ZR和精胺(Spm)、亚精胺(Spd)、腐胺(Put)含量及吲哚乙酸氧化酶(IAAO)、多酚氧化酶(PPO)活性呈现“升高-下降-升高”的趋势。相关性分析可知,Put与IAA/ABA、Spm以及IAAO与GA_(3)存在极显著正相关性(p<0.01),Spm与IAA/ABA、Spd,Put与Spd、POD以及IAAO与PPO呈显著正相关性(p<0.05),Spd与ABA含量存在极显著负相关性(p<0.01)。【结论】一定范围内的IAA、ABA含量、IAA/ABA和IAA/ZR升高及GA_(3)、ZR含量降低有利于不定根诱导分化和生长发育,后期组培苗体内Spm、Spd、Put含量的升高有利于不定根的形成,IAAO、PPO、POD酶活性变化与不定根的发生和伸长也有密切联系。【Objective】Muskmelon is one of the most important fruits in the world,which plays an important role in the adjustment of agricultural planting structure in China.But the narrow genetic basis,the lack of excellent germplasm resources,the long cycle and low efficiency of traditional breeding technology lead to the slow renewal of excellent varieties,which restricts the sustainable development of muskmelon industry.As a breeding method that can quickly and effectively obtain homozygous germplasm,the double monomer culture technology has become a research hotspot for muskmelon breeding workers at home and abroad.Rooting induction culture is an important part of the double haploid tissue culture system,which is directly related to the survival rate and seedling quality of tissue culture plantlets.There are many internal and external factors affecting the rooting process,including endogenous hormones,polyamines,related oxidases and exogenous substances such as ethylene and salicylic acid.The study aimed at investigating the changes of endogenous hormones,polyamine contents and related oxidase activities in the rooting culture process of melon double haploid shoots,in order to clarify the physiological mechanism of the response to adventitious root induction,differentiation and elongation,and to provide reference for regulating the rapid rooting of melon double haploid tissue culture plantlets.【Methods】The unfertilized ovary of thick-skinned muskmelon hybrids Jinxiucuiyu was cultured on MS+0.03 mg·L^(-1) TDZ+0.2 mg·L^(-1) KT+0.01 mg·L^(-1) IAA+15%coconut juice medium to obtain haploid buds,and then doubled with 700 mg·L^(-1) colchicines,after identification by flow cytometry,the double haploid buds with the same growth were selected and transfered onto MS+0.1 mg·L^(-1) IAA+30 g·L^(-1) sucrose+6 g·L^(-1) agar medium(pH=5.86)for rooting culture,all of were incubated at(25±2)℃,under a 16 h light/8 h dark cycle and 3000-4000 lx light intensity,the contents of endogenous hormones,polyamines and related oxid

关 键 词：甜瓜 双单倍体芽 组培生根 内源激素 多胺 关联酶 

分 类 号：S652[农业科学—果树学]