一株高效溶磷产红青霉培养条件优化及其溶磷特性  

Phosphate-solubilizing Properties and Optimization of Cultivation Conditions of Penicillium rubens:A Highly Efficient Phosphate Solubilizer

作　　者：赵光绪 杨合同 邵晓波[1] 崔志豪 刘红光 张杰[1] ZHAO Guang-xu;YANG He-tong;SHAO Xiao-bo;CUI Zhi-hao;LIU Hong-guang;ZHANG Jie(State Key Laboratory of Bio-based Materials and Green Paper,College of Bioengineering,Qilu University of Technology(Shandong Academy of Sciences),Jinan 250000;Zhucheng Zhongwo Biological Engineering Co.Ltd.,Weifang 262200)

机构地区：[1]齐鲁工业大学(山东省科学院)生物基材料与绿色造纸国家重点实验室,济南250000 [2]诸城市中沃生物工程有限公司,潍坊262200

出　　处：《生物技术通报》2023年第9期71-83,共13页Biotechnology Bulletin

基　　金：齐鲁工业大学(山东省科学院)创新项目(2022JBZ01-06);山东省自然科学基金(ZR2020KE038);国家自然科学基金项目(51978347)。

摘　　要：为了减少农业生产中化学肥料的使用,本研究利用无机磷培养基对富磷的茶树(Camellia sinensis L.)根际微生物进行筛选,获得一株对磷酸三钙具有高效降解能力的真菌菌株JL-1,经鉴定为产红青霉(Penicillium rubens)。通过检测菌株JL-1在溶磷过程中发酵液的pH值、磷含量和有机酸含量变化发现,该菌株在无机磷培养基中,发酵液pH值与葡萄糖酸含量、pH值与磷含量以及葡萄糖酸与磷含量分别呈显著负相关、显著负相关和显著正相关,且在电子显微镜下观察到磷酸三钙颗粒表面存在被侵蚀痕迹,深入分析发现菌株JL-1通过分泌葡糖糖酸实现侵蚀磷酸三钙与溶磷的目的。通过单因素试验、Plackett-Burman设计试验、最陡爬坡试验和中心组合试验等一系列试验对培养条件进行优化发现,菌株JL-1溶解磷酸三钙的最佳碳源和氮源分别是葡萄糖和硫酸铵。葡萄糖含量、磷酸三钙含量和温度是影响菌株JL-1溶磷能力的主要因素,在葡萄糖29.8 g/L,磷酸三钙7.1 g/L,温度31.9℃的条件下,菌株JL-1在无机磷培养基中的溶磷能力达到最佳,溶磷量达到1194.15 mg/L,是初始值的近3倍。在缺磷土壤中,该菌株能显著促进小麦(Triticum aestivum L.)生长,其根长、株高和总鲜重分别提高57.9%、36.4%和42.9%,进一步说明产红青霉JL-1具有良好的解磷、促生功能。菌株JL-1优良的溶磷特性与溶磷能力为其在生物肥料方向的开发和应用提供了参考。In order to reduce the use of chemical fertilizers in agricultural production,a fungal strain JL-1 with high efficiency in degrading tricalcium phosphate was isolated from phosphorus-rich tea(Camellia sinensis L.)rhizosphere soil by using inorganic phosphorus screening medium,and identified as Penicillium rubens.The changes of pH value,phosphorus content and organic acid content of fermentation broth of strain JL-1 in the process of phosphorus solubilization showed that there was a significant negative correlation between pH value and gluconic acid content,a significant negative correlation between pH value and phosphorus content,and a significant positive correlation between gluconic acid and phosphorus content in the fermentation broth of strain JL-1.And the surface erosion marks of tricalcium phosphate particles were observed under the electron microscope.Further analysis uncovered that strain JL-1 eroded tricalcium phosphate and dissolved phosphorus by secreting gluconic acid.The culture conditions were optimized through a series of experiments including single factor experiment,Plackett-Burman design experiemnt,steepest climb experiment and central combination experiment.The results showed that glucose and ammonium sulfate were the optimal carbon and nitrogen sources for dissovling tricalcium phosphate by strain JL-1.Glucose content,tricalcium phosphate content and temperature were significant factors affecting the phosphorus solubility of strain JL-1.Under the conditions of 29.8 g/L glucose,7.1 g/L tricalcium phosphate and 31.9℃,the phosphorus solubility of strain JL-1 in inorganic phosphorus medium,the solubilized phosphorus of the strain reached 1194.15 mg/L,which was nearly three times of the initial value.In phosphorus-deficient soil,this strain significantly promoted the growth of T.aestivum L.,with its root length,plant height and total fresh weight increased by 57.9%,36.4%and 42.9%respectively,indicating that the strain JL-1 had good functions of phosphate-dissolving and growth promotion.With the e

关 键 词：产红青霉 茶树根际 溶磷作用 磷酸三钙 葡萄糖酸 响应面优化 促生 

分 类 号：S144[农业科学—肥料学] Q939.9[农业科学—农业基础科学]