不同抗性烟草品系Rubisco及其活化酶对赤星病胁迫的响应  

Responses of Rubisco and Rubisco Activase in Different Resistant Tobacco Strains to Brown Spot Stress

作　　者：杨志晓 侯骞 刘国权 卢志刚 曹毅 芶剑渝 王轶 林英超 YANG Zhi-xiao;HOU Qian;LIU Guo-quan;LU Zhi-gang;CAO Yi;GOU Jian-yu;WANG Yi;LIN Ying-chao(Guizhou Academy of Tobacco Science,Guiyang 550081;College of Agronomy,Northwest A&F University,Yangling 712100;Tongren Branch of Guizhou Province Tobacco Company,Tongren 554300;Zunyi Branch of Guizhou Province Tobacco Company,Zunyi 563000)

机构地区：[1]贵州省烟草科学研究院,贵阳550081 [2]西北农林科技大学农学院,杨凌712100 [3]贵州省烟草公司铜仁市公司,铜仁554300 [4]贵州省烟草公司遵义市公司,遵义563000

出　　处：《生物技术通报》2023年第9期202-212,共11页Biotechnology Bulletin

基　　金：国家自然科学基金项目(32160648);贵州省科学技术基金项目([2020]1Y106,ZK[2021]一般110);中国烟草总公司科技重大专项[11020200027(JY-10)];贵州省公司科技项目(2022XM05),遵义市烟草公司科技项目(2021XM05)。

摘　　要：本研究以抗、感烟草品系JYH、CBH为试验材料,分析赤星病胁迫对光合作用和核酮糖-1,5-二磷酸核酮糖羧化酶/加氧酶(Rubisco)、核酮糖-1,5-二磷酸核酮糖羧化酶/加氧酶活化酶(RCA)活性及基因表达的影响。结果表明,胁迫9 d,JYH的光合作用及Rubisco、RCA活性、基因表达水平和蛋白含量受到明显抑制。赤星病胁迫显著降低CBH的光合作用及Rubisco、RCA活性和蛋白含量,Rubisco大、小亚基基因(rbcL、rbcS)、RCA基因(rca)的相对表达量也持续下降。JYH的Rubisco、RCA活性、基因表达水平和蛋白含量均显著高于CBH。相关性分析结果显示,Pn与Rubisco、RCA活性及基因表达量呈显著、极显著正相关。在赤星病胁迫下,JYH可维持较高的Pn与Rubisco、RCA活性,从而具有较强抗性。Using resistant and susceptible tobacco strains JYH and CBH as test materials,the effects of brown spot stress on the photosynthesis and activities of ribulose-1,5-bisphosphate carboxylase/oxygenase(Rubisco)and ribulose-1,5-diphosphate carboxylase/oxygenase activase(RCA)and their gene expressions were analyzed.The results showed that,there were obvious inhibition effects on a series of photosynthetic parameters and the activities,expression levels and protein contents of Rubisco and RCA in JYH at 9 d of brown spot stress.Meanwhile,tobacco brown spot stress notably reduced the photosynthesis and activities of Rubisco and RCA in CBH,in addition,the relative expression of Rubisco large and small subunits genes(rbcL and rbcS)and RCA genes(rca)and their protein contents all decreased under stress condition.In comparison with CBH,the activities,expression levels and protein contents of Rubisco and RCA in JYH were all significantly higher at the stress treatment of tobacco brown spot.The correlation analysis results also indicated that Pn had significant and extremely significant positive correlation with the activities,gene expression of Rubisco and RCA,respectively.These results suggest that the resistant strain JYH can maintain higher values of Pn,Rubisco and RCA activities under tobacco brown spot stress,which account for the resistance to brown spot stress.

关 键 词：烟草赤星病 光合作用 1 5-二磷酸核酮糖羧化酶/加氧酶 1 5-二磷酸核酮糖羧化酶/加氧酶活化酶 基因表达 相关性分析 抗性差异 品系 

分 类 号：S435.72[农业科学—农业昆虫与害虫防治]