臭氧对MBBR硝化效率及副溶血弧菌杀菌效果影响  

Effect of ozone on nitrification efficiency of MBBR and bactericidal effect of Vibrio parahaemolyticus

作　　者：石婷宇 王国庆 胡鑫 谭洪新[1,2,3] 刘文畅 罗国芝[1,2,3] SHI Tingyu;WANG Guoqing;HU Xin;TAN Hongxin;LIU Wenchang;LUO Guozhi(Shanghai Engineering Research Center of Aquaculture,Shanghai Ocean University,Shanghai 201306,China;Shanghai Collaborative Innovation Center for Cultivating Elite Breeds and Green-culture of Aquaculture Animals,Shanghai 201306,China;Key Laboratory of Freshwater Aquatic Germplasm Resources,Ministry of Agriculture and Rural Affairs,Shanghai Ocean University,Shanghai 201306,China)

机构地区：[1]上海海洋大学上海水产养殖工程技术研究中心,上海201306 [2]上海市水产动物良种创制与绿色协同创新中心,上海201306 [3]上海海洋大学水产科学国家级实验教学示范中心,上海201306

出　　处：《渔业现代化》2023年第5期25-32,共8页Fishery Modernization

基　　金：上海市科学技术委员地方院校能力提升项目(23010502300)。

摘　　要：臭氧作为一种强氧化剂,常用作移动床生物膜反应器(Moving Bed Biofilm Reactor,MBBR)系统的杀菌消毒剂。为了得到臭氧对MBBR处理水质的影响与对副溶血弧菌的杀菌效果,用10 L反应器,在60%填充率下,研究不同质量浓度臭氧(0、1、2、4、6 mg/L)对系统硝化能力与对副溶血弧菌(Vibrio parahaemolyticus)的杀灭效果。结果显示:与对照组相比,2 mg/L臭氧对滤料细胞死亡率影响不显著(P=0.49);总氨氮(TAN)去除率分别为55.11%±5.32%、57.89%±9.01%、49.2%±5.99%、33.07%±2.68%、34.22%±8.89%;当臭氧通入量在4 mg/L及以上时,TAN去除率显著低于其余组(P<0.05);臭氧剂量2 mg/L及以上时,TCBS平板上未检测到副溶血弧菌(从108 CFU/mL降低到不能被检测出的水平)。综合考虑臭氧对滤料细胞死亡率、氨氮处理效率以及副溶血弧菌的杀菌率,MBBR池臭氧推荐使用质量浓度为2 mg/L。As a strong oxidant,ozone is often used as a bactericidal disinfectant for MBBR systems.In order to obtain the effect of ozone on the water quality of MBBR treatment and on the bactericidal rate of Vibrio parahaemolyticus,a 10 L reactor was used to determine the nitrification capacity of the system and the number of viable dead cells of the biofilm samples under a 10 L reactor at 60%filling rate to study the effects of different concentrations of ozone(0,1,2,4,6 mg/L)on the nitrification capacity of the system and the killing of Vibrio parahaemolyticus.The results showed that 2 mg/L ozone had no significant effect on cell mortality in biofilm sample(P=0.49).The removal rates of TAN were 55.11%±5.32%,57.89%±9.01%,49.2%±5.99%,33.07%±2.68%,34.22%±8.89%,and the 4 mg/L ozone dose group was significantly lower than that of 0,1,2 mg/L ozone dose group(P=0.042).At ozone doses above 2 mg/L,Vibrio parahaemolyticus was not detected on TCBS plates.This study showed that according to the mortality rate of biofilm cells,the treatment efficiency of ammonia nitrogen and the sterilization rate of Vibrio parahaemolyticus.The 2 mg/L ozone can effectively kill Vibrio parahaemolyticus and do not affect the nitration reaction efficiency of MBBR.

关 键 词：MBBR 臭氧 硝化 副溶血弧菌 循环水养殖系统 

分 类 号：S966.12[农业科学—水产养殖]