内蒙古自治区通辽市部分规模化牛场BCoV流行病学调查  

Epidemiological Investigation of BCoV in Some Large-scale Cattle Farms in Tongliao City, Inner Mongolia Autonomous Region

作　　者：石晓娇 谢梦圆 张园园 郭宇 萨茹拉 陈柯佳 尚和卫 郝彦儒 许燕飞 徐晓静[1] SHI Xiaojiao;XIE Mengyuan;ZHANG Yuanyuan;GUO Yu;SA Rula;CHEN Kejia;SHANG Hewei;HAO Yanru;XU Yanfei;XU Xiaojing(College of Veterinary Medicine,Inner Mongolia Agricultural University,Hohhot 010018,China;Inner Mongolia Animal Agriculture Association,Hohhot O10000,China;Inner Mongolia Animal Disease Control Center,Hohhot 010020,China)

机构地区：[1]内蒙古农业大学兽医学院,内蒙古呼和浩特010018 [2]内蒙古畜牧业协会,内蒙古呼和浩特010000 [3]内蒙古自治区动物疫病预防控制中心,内蒙古呼和浩特010020

出　　处：《兽医导刊》2023年第2期20-23,共4页Veterinary Orientation

基　　金：呼和浩特市重大科技专项(2022-农-重-1-1);呼和浩特市科技创新领域人才项目(2022RC-联合体-1);内蒙古自治区科技重大专项“牛腹泻疾病防控关键技术研发与标准化操作规程示范推广”(2020ZD0006);内蒙古自治区科技重大专项“牛羊支原体肺炎和病毒性呼吸道疾病的防控技术研发与示范”(2021ZD0013)。

摘　　要：从内蒙古自治区通辽市部分规模化养牛场采集粪样350份、血样325份,分别采用RT-PCR和ELISA方法进行牛冠状病毒(Bovine coronavirus,BCoV)的分子流行病学调查,了解内蒙古自治区通辽市规模化养殖场牛冠状病毒的流行状况,以期为通辽地区养牛场牛冠状病毒病的防控提供参考。结果显示:抗原总阳性率为22.86%,抗体总阳性率为7.69%。在采集的样品中,0~2月龄犊牛的抗原阳性率最高,达57.50%,抗体阳性率最高,达19.00%,表明通辽地区牛冠状病毒流行率较高,及时采取科学有效的防控措施是很有必要的。In order to understand the epidemic situation of Bovine coronavirus disease in large-scale farms in Tongliao City,Inner Mongolia Autonomous Region,350 manure samples and 325 serum samples were collected from some large-scale cattle farms in Tongliao City,Inner Mongolia Autonomous Region.Molecular epidemiological investigation of Bovine coronavirus(BCoV)was performed using RT-PCR and ELISA methods for fecal samples and serum,respectively.The results showed that the total positive rate of antibody was 7.69%,and the total positive rate of antigen was 22.86%.Among the samples collected,calves aged 0~2 months had the highest antigen positivity rate,reaching 57.50%,and antibody positivity rate was the highest,reaching 19.00%,indicating that the prevalence of bovine coronavirus in Tongliao City was high,and it was necessary to take scientific and effective prevention and control measures in time.

关 键 词：牛冠状病毒 流行病学调查 ELISA RT-PCR 

分 类 号：S858.23[农业科学—临床兽医学]