裸花紫珠醇提物UPLC指纹图谱建立及抗呼吸道合胞病毒谱效关系研究  

Establishment of UPLC fingerprint and study on anti-respiratory syncytial virus spectrum-effective relationship of ethanol extract from Callicarpa nudiflora

作　　者：邵冰梅 李小锋 闫蓓蓓 刘启峰 秦焕云 赵祥君 侯林 SHAO Bingmei;LI Xiaofeng;YAN Beibei;LIU Qifeng;QIN Huanyun;ZHAO Xiangjun;HOU Lin(Qingdao Academy of Chinese Medicinal Sciences,Shandong University of Traditional Chinese Medicine,Shandong Qingdao 266000,China;National Enterprise Technology Center,Jiangxi Puzheng PharmaceuticalCo.,Ltd.,Jiangxi Jiʼan 343100,China;School of Pharmacy,Shandong University of Traditional ChineseMedicine,Jinan 250300,China;Dept.of Neurosurgery,Tai’an Municipal Hospital,Shandong Tai’an271000,China)

机构地区：[1]山东中医药大学青岛中医药科学院,山东青岛266000 [2]江西普正制药股份有限公司国家企业技术中心,江西吉安343100 [3]山东中医药大学药学院,济南250300 [4]泰安市立医院神经外科,山东泰安271000

出　　处：《中国药房》2023年第20期2465-2470,共6页China Pharmacy

基　　金：山东省重点研发计划(重大科技创新工程)项目(No.2021CXGC010511,No.2020CXGC010505)。

摘　　要：目的建立裸花紫珠醇提物的指纹图谱,分析其体外抗呼吸道合胞病毒(RSV)活性并进行谱效关系研究。方法以10%、30%、50%、70%、90%乙醇为溶剂,制备4批裸花紫珠药材的20批醇提物。采用超高效液相色谱法,利用《中药色谱指纹图谱相似度评价系统(2012版)》绘制20批裸花紫珠醇提物的指纹图谱并进行相似度评价;采用致细胞病变效应法和MTT法考察裸花紫珠醇提物对RSV的体外抑制活性;运用Pearson相关性分析、灰色关联度分析、正交偏最小二乘(OPLS)分析进行谱效关系研究。结果20批裸花紫珠醇提物有25个共有峰,相似度为0.912~0.998,共有峰峰面积的RSD为33.54%~162.28%。20批裸花紫珠醇提物对RSV的平均半数抑制浓度为9.55~272.23μg/mL;Pearson相关性分析、灰色关联度分析、OPLS分析结果表明,共有峰8、10、12、16、18~19、22~24与药效指标的Pearson相关系数(P<0.05)和回归系数均为负值,关联度均大于0.6,变量重要性投影值均大于1。结论20批裸花紫珠醇提物成分相似但含量差异明显,且具有不同程度的体外抗RSV活性,其中共有峰8、10、12、16、18~19、22~24对应成分可能是裸花紫珠抗RSV的特征成分。OBJECTIVE To establish the fingerprint of the ethanol extract from Callicarpa nudiflora,analyze its antirespiratory syncytial virus(RSV)activity in vitro,and study the relationship between spectrum and effect.METHODS Using 10%,30%,50%,70%and 90%ethanol as solvent,20 batches of ethanol extracts from 4 batches of C.nudiflora were prepared.The fingerprints for 20 batches of ethanol extracts from C.nudiflora were mapped by ultra-high-performance liquid chromatography(UPLC),and the similarity evaluation was conducted by using the Similarity Evaluation System for Traditional Chinese Medicine Chromatographic Fingerprints(2012 edition).The cytopathic effect method and MTT method were used to investigate the in vitro inhibitory activity of the ethanol extracts from C.nudiflora on RSV.Pearson correlation analysis,grey correlation degree and orthogonal partial least squares(OPLS)analysis were used to study the spectrum-effect relationship.RESULTS There were 25 common peaks in 20 batches of ethanol extracts from C.nudiflora,and the similarities ranged from 0.912 to 0.998,and the RSDs of common peak areas were 33.54%-162.28%.The average values of IC50 for RSV of 20 batches of ethanol extracts from C.nudiflora were 9.55-272.23μg/mL.The results of Pearson correlation analysis,grey correlation analysis and OPLS analysis showed that the Pearson correlation coefficients(P<0.05)of the common peaks 8,10,12,16,18-19,22-24 with pharmacodynamic indicators and regression coefficients were all negative,the correlation coefficients were all greater than 0.6,and the values of variable importance in projection were all greater than 1.CONCLUSIONS Twenty batches of ethanol extracts from C.nudiflora have similar components but significant differences in content,and exhibit different degrees of antiRSV activity in vitro.The corresponding components of common peaks 8,10,12,16,18-19,22-24 may be the characteristic components of anti-RSV of C.nudiflora.

关 键 词：裸花紫珠 醇提物 呼吸道合胞病毒 指纹图谱 抗病毒 谱效关系 

分 类 号：R917[医药卫生—药物分析学] R978.7[医药卫生—药学]