基于“来源-成分-靶点-药效”关联的枳实导滞丸质控指标探讨  

Study on the quality of Zhishi Daozhi Pills based on the relations among species,ingredient,target and efficacy

作　　者：刘永富 熊英[1] 刘佳 赵香玉 赖晓微 赖宇文 邓可众[1] LIU Yongfu;XIONG Ying;LIU Jia;ZHAO Xiangyu;LAI Xiaowei;LAI Yuwen;DENG Kezhong(College of Pharmacy,Jiangxi University of Chinese Medicine,Jiangxi Nanchang 330004,China)

机构地区：[1]江西中医药大学药学院,江西南昌330004

出　　处：《中国医院药学杂志》2023年第18期2009-2015,共7页Chinese Journal of Hospital Pharmacy

基　　金：国家自然科学基金资助项目(编号:81960709);江西省大学生创新创业计划(编号:s202101412018)。

摘　　要：目的:探讨枳实导滞丸的质控指标,为该药的质量评价提供参考。方法:以食积证模型中小鼠的胃残留率、肠推进率及血清中胃肠激素水平[胃动素(MTL)、胃泌素(GAS)、活性肠肽(VIP)]为指标分别评价酸橙枳实和甜橙枳实所制枳实导滞丸的药效;建立高效液相色谱法同时测定枳实导滞丸中芸香柚皮苷、柚皮苷、橙皮苷及新橙皮苷的含量;检索TCMSP、Swiss Target Prediction和Genecards数据库,查找4种黄酮苷治疗食积证有关靶点,分析“来源-成分-靶点-药效”之间的关联性。结果:酸橙枳实和甜橙枳实制备的枳实导滞丸对食积证模型小鼠均有显著疗效;靶点分析表明,4个黄酮苷及苷元均可调节胃肠运动;2种丸剂中黄酮苷成分差异显著,酸橙枳实制备的丸剂中橙皮苷含量低于甜橙枳实制备的丸剂。结论:高效液相色谱法同时测定丸剂中的4个黄酮苷含量准确便捷,建议采用高效液相法同时测定芸香柚皮苷、柚皮苷、橙皮苷及新橙皮苷的总量作为枳实导滞丸的质量控制指标,有利于全面评价不同来源枳实制备的枳实导滞丸质量。OBJECTIVE To explore the quality control indicators of Zhishi Daozhi Pills and provide reference for the quality evaluation of the drug.METHODS The gastric residual rate,the intestinal propulsion rates and the gastrointestinal hormones(MTL,GAS,VIP)of mice with syndrome of food retention were used as indexes to evaluate the efficacy of Zhizhi Daozhi Pills prepared from different Aurantii Fructus Immaturus(from Citrus aurantium L.or C.Citrus sinensis)respectively.The HPLC method was established for the simultaneous determination of narirutin,naringin,hesperidin and neohesperidin in Zhishi Daozhi Pills.The targets of flavonoid glycosides and their aglycones for syndrome of food retention were studied by searching TCMSP,Swiss Target Prediction and Genecards databases,the association between"source-component-targets-efficacy"was analyzed.RESULTS All of the Zhishi Daozhi Pills prepared from different Aurantii Fructus Immaturus had significant efficacy on mice with syndrome of food retention.Simultaneous determinations of four flavonoid glycosides in pills by HPLC were accurate and convenient.There were significant differences in flavonoid glycosides between the two kinds of pills.The contents of hesperidin in the pills which contained Aurantii Fructus Immaturus from C.Citrus aurantium were much lower than the others.The analyses on targets showed that all of the flavonoids and aglycones could regulate gastrointestinal motility.CONCLUSION It is suggested that the total amount of narirutin,naringin,hesperidin and neohesperidin should be used as the quality control index of Zhishi Daozhi Pills.HPLC is helpful to accurately evaluate the quality of Zhishi Daozhi Pills prepared from different Aurantii Fructus Immaturus.

关 键 词：枳实导滞丸 不同品种 含量测定 药效 靶点 黄酮苷 质量控制 

分 类 号：R283.6[医药卫生—中药学]