人参皂苷Rg1对脑出血大鼠神经功能恢复作用中凋亡相关因子的影响  

Effect of ginsenoside Rg1 on apoptosis-related factors in the recovery of neurological function in rats with intracerebral hemorrhage

作　　者：苏男 巩涛 庞海[1] 陈玉玲 李梁[1] 石素琴 蔡志平[1] SU Nan;GONG Tao;PANG Hai;CHEN Yuling;LI Liang;SHI Suqin;CAI Zhiping(Baotou Medical College,Inner Mongolia University of Science and Technology,Baotou 014040,China;Bayannur City Hospital,Inner Mongolia;The Second Affiliated Hospital of Baotou Medical College,Inner Mongolia University of Science and Technology)

机构地区：[1]内蒙古科技大学包头医学院,内蒙古包头014040 [2]内蒙古巴彦淖尔市医院 [3]内蒙古科技大学包头医学院第二附属医院

出　　处：《包头医学院学报》2023年第10期6-10,共5页Journal of Baotou Medical College

基　　金：包头医学院科学研究基金(2020LH03001)。

摘　　要：目的:探讨大鼠脑出血后注射人参皂苷Rg1促进功能恢复机制中有无凋亡相关因子的参与。方法:大鼠随机分为3组,对照组大鼠脑立体定位下于内囊内侧注射生理盐水1μL,模型组注射Ⅶ型胶原酶1μL,术后1 d、7 d、14 d进行大鼠运动功能评分;人参皂苷组于造模后次日腹腔注射人参皂苷Rg1;7 d后测定大鼠脑含水量,脑组织进行免疫组化观察Bcl-2、Bax的表达。结果:脑出血模型组内囊周围神经细胞有明显细胞形态受损,细胞边界不清,结构移位受压;在1 d、7 d、14 d时,模型组大鼠神经功能评分高于对照组(P<0.05),人参皂苷Rg1组大鼠在7 d、14 d时低于模型组(P<0.05);7 d时脑含水量测定模型组高于对照组(P<0.05),人参皂苷Rg1组低于模型组(P<0.05);模型组和人参皂苷Rg1组大鼠内囊区周围的Bax阳性神经元数量高于对照组(P<0.05);Bcl-2阳性细胞数量在模型组大鼠脑切片中低于对照组,人参皂苷Rg1组高于模型组,低于对照组(P<0.05)。结论:大鼠脑出血后注射人参皂苷Rg1,有利于大鼠运动功能恢复;相关脑区的Bcl-2阳性细胞增加。Objective:To analyze the participation of apoptosis-related factors in the mechanism of ginsenoside Rg1 promoting functional recovery after intracerebral hemorrhage in rats.Methods:The rats were randomly divided into three groups,while the rats in the control group were injected with 1μL of normal saline into the inner capsule under stereotaxic localization,and the rats in the model group were injected with 1μL of typeⅦcollagenase.The motor function of the rats was scored on 1st,7th and 14th day after operation.The ginsenoside group was intraperitoneally injected with ginsenoside Rg1 on the next day after modeling.The brain water content of rats was measured on the 7th day after operation,and the expression of Bcl-2 and Bax in brain tissue was observed by immunohistochemistry.Results:In the model group of intracerebral hemorrhage,there were significant cell morphology damage,unclear cell boundaries,and structural displacement and compression of the nerve cells around the inner capsule;On the 1st,7th and 14th day,the neurological function score of the model group was higher than that of the control group(P<0.05),and that in the ginsenoside Rg1 group was lower than that in the model group on the 7th and 14th day(P<0.05);On the 7th day,the brain water content in the model group was higher than that in the control group(P<0.05),and that in the ginsenoside Rg1 group was lower than that in the model group(P<0.05);The number of Bax positive neurons around the inner capsule area in the model group and ginsenoside Rg1 group was more than that in the control group(P<0.05);The number of Bcl-2 positive cells in brain slices of rats in the model group was lower than that in the control group,and that in the ginsenoside Rg1 group was higher than that in the model group and lower than that in the control group(P<0.05).Conclusion:The injection of ginsenoside Rg1 after intracerebral hemorrhage in rats is beneficial to the recovery of motor function,and the increase of Bcl-2 positive cells in related brain regions.

关 键 词：脑出血 大鼠 神经功能 BCL-2蛋白 BAX蛋白 

分 类 号：R285.5[医药卫生—中药学]