谷氨酸铁的制备及表征  

Preparation and characterization of glutamate iron chelate

作　　者：刘新硕 王真 李晴 杨文智 李海鹰 LIU Xinshuo;WANG Zhen;LI Qing;YANG Wenzhi;LI Haiying(Key Laboratory of Pharmaceutical Quality Control of Hebei Province,College of Pharmaceutical Sciences,Hebei University,Baoding 071002,China)

机构地区：[1]河北大学药学院,河北省药物质量分析控制重点实验室,河北保定071002

出　　处：《河北大学学报（自然科学版）》2023年第5期476-483,共8页Journal of Hebei University(Natural Science Edition)

基　　金：河北省自然科学基金资助项目(C2021201026);河北省大学生科技创新能力培育专项(22E50036D)。

摘　　要：将谷氨酸(Glu)与三价铁离子(Fe^(3+))螯合,制备谷氨酸铁螯合物Glu-Fe(Ⅲ),并采用傅里叶变换红外光谱(FT-IR)、差示扫描量热(DSC)和热重(TGA)分析法对其结构进行表征,证实Glu与Fe^(3+)成功发生螯合反应.采用间接碘量法测定Glu-Fe(Ⅲ)中的Fe^(3+)质量分数,并以其为评价指标,通过单因素实验和Box-Benhnken设计(BBD),考察Glu和Fe^(3+)的物质的量比及反应pH、时间、温度4个因素对制备Glu-Fe(Ⅲ)螯合物中Fe^(3+)质量分数的影响.最终在Glu与Fe^(3+)物质的量比为3∶5,反应pH为4.8、温度为50℃和反应时间为3 h的条件下,获得Fe^(3+)质量分数为19.91%的Glu-Fe(Ⅲ)螯合物.制备出的Glu-Fe(Ⅲ)可作为同时补充谷氨酸和铁的营养强化剂.Glutamic acid(Glu)was reacted with trivalent iron ions(Fe ^(3+))to prepare glutamate iron chelate[Glu-Fe(Ⅲ)].Glu-Fe(Ⅲ)was characterized by FT-IR,DSC,and TGA analysis.The Fe ^(3+)content in Glu-Fe(Ⅲ)was determined by an indirect iodometric method and identified as an evaluation index for optimizing the preparation condition of Glu-Fe(Ⅲ).The effects of molar ratio of glutamic acid to iron,pH,time and temperature on the iron content of Glu-Fe(Ⅲ)were investigated by single factor method and Box-Benhnken design(BBD).The optimum preparation condition of Glu-Fe(Ⅲ)was Glu/Fe ^(3+)molar ratio of 3∶5,pH 4.8,50℃and 3 h,and the iron content of Glu-Fe(Ⅲ)prepared under the optimized conditions was 19.91%.Glu-Fe(Ⅲ)could be a new iron and glutamic acid nutrient supplement.

关 键 词：谷氨酸铁螯合物 制备工艺 Box-Benhnken设计 表征 营养强化剂 

分 类 号：O634[理学—高分子化学]