HPLC法同时测定湘莲中芦丁、金丝桃苷及槲皮素含量  

Determination of rutin,hyperoside,quercetin in Xiang lotus by HPLC

作　　者：钟菲菲 李静 雷德卿 杨韵 伍健芳 欧阳丽 ZHONG Feifei;LI Jing;LEI Deqing;YANG Yun;WU Jianfang;OUYANG Li(Changsha Institute for Food and Drug Control,Changsha,Hunan 410008,China)

机构地区：[1]长沙市食品药品检验所,湖南长沙410008

出　　处：《食品与机械》2023年第9期57-64,共8页Food and Machinery

基　　金：湖南省自然科学基金项目(编号:2021JJ80076)。

摘　　要：目的:建立高效液相色谱法同时测定湘莲中芦丁、金丝桃苷及槲皮素的含量。方法:采用Agilent ZORBAX SB-C_(18)色谱柱(4.6 mm×250 mm,5μm),以甲醇—0.6%磷酸水溶液为流动相,梯度洗脱,流速1.0 mL/min,柱温30℃,检测波长255 nm。结果:20批湘莲样品中芦丁、金丝桃苷及槲皮素含量分别为1.32~2.06,1.36~2.43,1.41~3.26 mg/kg;芦丁、金丝桃苷和槲皮素检测质量浓度线性范围均为5~200μg/mL(R^(2)=0.9999),在相应的线性范围内,3种黄酮类物质线性关系好,灵敏度高,精密度、稳定性、重复性及回收率试验相对标准偏差(RSD)均小于4.0%;芦丁、金丝桃苷及槲皮素的平均加标回收率分别为96.6%,96.9%,97.4%。结论:该方法操作便捷且结果准确,可用于湘莲中黄酮类成分的含量测定和质量评价。Objective:To establish a HPLC method for simultaneous determination of rutin,hyperoside,quercetin in Xiang lotus.Methods:The HPLC method was performed on an Agilent ZORBAX SB-C_(18) column(4.6 mm×250 mm,5 m)with gradient elution of methanol and 0.6%phosphoric acid solution.The flow rate was 1.0 mL/min.The column temperature was 30℃and the detection wavelength was set at 255 nm.Results:The contents of rutin,hypericin and quercetin in 20 batches of lotus samples were 1.32~2.06,1.36~2.43,1.41~3.26 mg/kg,respectively.The linear range of the concentration of rutin,hypericin and quercetin was 5~200 g/mL(R^(2)=0.9999),the three flavonoids showed a good linearity,a high sensitivity,and good serviceability.The relative standard deviation(RSD)for precision,stability,repeatability,and recoveries test were all less than 4.0%,and the average recovery rate of added standard were 96.6%,96.9%,97.4%,respecitvely.Conclusion:The method is convenient and accurate,and can be used for the content determination and quality evaluation of flavonoids in Xiang lotus.

关 键 词：湘莲 高效液相色谱 芦丁 金丝桃苷 槲皮素 

分 类 号：O657.72[理学—分析化学] TS207.3[理学—化学]