亚急性瘤胃酸中毒湖羊瘤胃液中菌群和B族维生素丰度变化的关联分析  

Correlation Analysis of Microflora and B Vitamins Abundance in Rumen Fluid of Hu Sheep in Subacute Rumen Acidosis

作　　者：吴建新 闫振兴 段宏伟 吕建树 曾建林 马小军[1,2] 胡俊杰 WU Jianxin;YAN Zhenxing;DUAN Hongwei;LYU Jianshu;ZENG Jianlin;MA Xiaojun;HU Junjie(College of Veterinary Medicine,Gansu Agricultural University,Lanzhou 730070,China;Gansu Key Laboratory of Animal Reproduction and Physiology and Reproductive Regulation,Lanzhou 730070,China)

机构地区：[1]甘肃农业大学动物医学院,兰州730070 [2]甘肃省动物生殖与生理及繁殖调控重点实验室,兰州730070

出　　处：《中国畜牧兽医》2023年第10期4045-4057,共13页China Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金(32160858)。

摘　　要：【目的】本试验旨在探讨湖羊亚急性瘤胃酸中毒(subacute rumen acidosis,SARA)对瘤胃液中微生物菌群结构和B族维生素表达丰度的影响,同时分析二者之间的相关性。【方法】选用体况良好的湖羊12只,随机均分为2组,每组6只,对照组饲料精粗比为3∶7,SARA组饲料精粗比为7∶3建立SARA模型。通过16S rRNA测序技术分析菌群结构变化,利用液相色谱-串联质谱(LC-MS)方法分析瘤胃液中B族维生素的表达丰度,并用Pearson法分析二者相关性。【结果】对照组湖羊瘤胃菌群多样性指数(Shannon和Chao1)显著高于SARA组,菌群多样性显著高于SARA组(P<0.05)。在细菌门水平,对照组放线菌门(Actinobacteria)、Candidatus_Saccharibacteria、软壁菌门(Tenericutes)丰度显著高于SARA组(P<0.05);在细菌属水平,对照组双歧杆菌属(Bifidobacterium)、夏普氏菌属(Sharpea)、产丁酸盐菌属(Saccharibacteria)、未分类的瘤胃球菌属(unclassified_Ruminococcaceae)显著高于SARA组(P<0.05);而未分类的脱硫弧菌属(unclassified_Desulfovibrionaceae)、未分类的梭菌属(unclassified_Clostridiales)和拟普雷沃氏菌属(Alloprevotella)显著低于SARA组(P<0.05)。对照组与SARA组筛选出VB 6(吡哆醛、吡哆醇、吡哆胺)、VB 3、VB 9及VB 126种差异代谢物,其表达丰度SARA组显著高于对照组(P<0.05)。Pearson相关性结果显示,6种差异B族维生素与绝大多数瘤胃微生物具有显著相关性(R>0或R<0)。【结论】SARA改变了瘤胃液中菌群门与属水平结构,导致菌群多样性降低,增加了VB 6、VB 3、VB 9及VB 12表达丰度,且与瘤胃液中大部分菌群具有显著相关性。【Objective】The purpose of this study was to investigate the effects of subacute rumen acidosis(SARA)on the microbial flora structure and B vitamins expression abundance in rumen fluid of Hu sheep,and to analyze the correlation between them.【Method】Twelve healthy Hu sheep were randomly divided into 2 groups,with 6 sheep in each group.The ratio of feed concentrate to forage in control group was 3∶7,and that in SARA group was 7∶3 to establish SARA model.The structural changes of bacteria were analyzed by 16S rRNA sequencing technology,the abundance of B vitamins expression in rumen fluid was analyzed by LC-MS method,and the correlation between them was analyzed by Pearson method.【Result】The rumen microbial diversity index(Shannon and Chao1)of Hu sheep in control group was significantly higher than that in SARA group,and the microbial diversity was significantly higher than that in SARA group(P<0.05).At the level of the phylum bacterialis,the abundance of Actinobacteria,Candidatus_Saccharibacteria and Tenericutes in control group were significantly higher than that in SARA group(P<0.05).At the genus level,Bifidobacterium,Sharpea,Saccharibacteria,unclassified_Ruminococcaceae in control group were significantly higher than that in SARA group(P<0.05),but unclassified_Desulfovibrionaceae,unclassified_Clostridiales,Alloprevotella were significantly lower than that in SARA group(P<0.05).Six different metabolites,VB 6(pyridoxal,pyridoxine,pyridoxine),VB 3,VB 9 and VB 12,were screened out from control group and SARA group,and their expression abundance in SARA group was significantly higher than that in control group(P<0.05).Pearson correlation results showed that six different B vitamins species were significantly associated with the vast majority of rumen microorganisms(R>0 or R<0).【Conclusion】SARA changed the phylum and genus level structure of bacteria in rumen fluid,resulting in decreased bacterial diversity and increased the abundance of VB 6,VB 3,VB 9 and VB 12 expression,which was significantly co

关 键 词：亚急性瘤胃酸中毒(SARA) 微生物 B族维生素 丰度 

分 类 号：S816[农业科学—饲料科学]