哈萨克羊杂交群体多胎基因检测分析  

Research and Analysis of Multifetal Gene Detection of Kazakh Sheep Hybrid Population

作　　者：张恺 玛尔孜娅•亚森 阿米妮古丽•阿不来孜 买坎•沙力 周喜荣[4] 于丽娟[2,3] 白锋 罗春彦 奴丽曼古丽•阿不拉 邸全文 张艳花 ZHANG Kai;MAERZIYA·Yasen;AMINIGULI·Abulizi;MAIKAN·Shali;ZHOU Xirong;YU Lijuan;BAI Feng;LUO Chunyan;NULIMANGULI·Abula;DI Quanwen;ZHANG Yanhua(College of Animal Science,Xinjiang Agricultural University,Urumqi 830052,China;Institute of Animal Science,Xinjiang Academy of Animal Sciences,Urumqi 830026,China;Key Laboratory of Evaluation and Utilization of Livestock and Poultry Resources(Sheep),Ministry of Agriculture and Rural Affairs,Urumqi 830026,China;Institute of Animal Husbandry Economics and Information,Xinjiang Academy of Animal Sciences,Urumqi 830026,China;Liushugou Township Agriculture(Animal Husbandry)Development Service Center,Hami,Xinjiang 839003,China;Qiaolak Tiereke Town of Tex County,Tex,Xinjiang 835500,China)

机构地区：[1]新疆农业大学动物科学学院,乌鲁木齐830052 [2]新疆畜牧科学院畜牧研究所,乌鲁木齐830026 [3]农业农村部畜禽资源(羊)评价利用重点实验室,乌鲁木齐830026 [4]新疆畜牧科学院畜牧业经济与信息研究所,乌鲁木齐830026 [5]哈密市伊州区柳树沟乡农业(畜牧业)发展服务中心,新疆哈密839003 [6]新疆伊犁特克斯县乔拉克铁热克镇,新疆特克斯835500

出　　处：《草食家畜》2023年第5期1-6,共6页Grass-Feeding Livestock

基　　金：中央引导地方科技发展专项“羊遗传资源评价与利用实验平台建设”(ZYYD2023B10);新疆维吾尔自治区产业技术体系项目“新疆牧区地方肉羊选育改良技术体系”(XJMQ-1)。

摘　　要：【目的】利用基因检测技术检测哈萨克羊杂交后代多胎主效基因FecB,进行基因分型,为多胎种羊选种留种提供依据。【方法】随机选取59只哈萨克羊杂交个体,采集血样提取DNA,利用限制性片段长度多态性聚合酶链反应(PCR-RFLP)技术检测FecB基因的多态性。【结果】基因分型统计显示,实验群体5%的母羊含FecB基因纯合子,47.5%为FecB基因杂合子,基因型频率分别为0.05,0.475,0.475。【结论】实验表明通过杂交导入小尾寒羊多胎基因,哈萨克羊杂交群体的多胎主效基因比例显著提高,有效提升了原有群体的繁殖率,同时利用基因检测技术,选择含有多胎基因的个体留种,对育种群体种羊进行精准选择,可为地方多胎品种(系)的培育提供技术支撑。【Objective】The aim was to improve the reproductive efficiency of Kazakh sheep by using genetic detection technology,detecting and genotyping the main effect gene FecB for multiple fetals in hybrid offspring,and providing a basis for selection and retention of multiple-born sheep.【Methods】59 individuals were randomly selected,blood samples were collected to extract DNA,and polymorphisms of the FecB genes were detected by restriction fragment length polymorphism polymerase chain reaction(PCRRFLP).【Results】Genotyping statistics showed that 5%of the ewes in the experimental population contained FecB gene homozygotes,47.5%were FecB gene heterozygotes,and the genotype frequencies were 0.05,0.475 and 0.475,respectively.【Conclusion】The experiment showed that the introduction of small-tailed cold sheep significantly increased the proportion of multiple major genes in the Kazakh sheep hybrid population,which can effectively improve the reproductive rate of the original population,and at the same time,the use of genetic detection technology to select individuals containing multiple genes to maintain the breed,and the accurate selection of breeding groups,can provide technical support for the breeding of local breeds of multiple breeds(lines).

关 键 词：哈萨克羊 多胎性状 多胎主效基因 小尾寒羊 聚合酶链式反应 

分 类 号：S826[农业科学—畜牧学]