牛源大肠杆菌的分离鉴定与耐药性分析  

Isolation,Identification and Drug Resistance Analysis of Bovine Escherichia coli

作　　者：梁纤纤 李威 郑启铭 李晓卓 张凌 刘文锴 韩翔舒 苏战强[1] 夏俊 LIANG Qianqian;LI Wei;ZHENG Qiming;LI Xiaozhuo;ZHANG Ling;LIU Wenkai;HAN Xiangshu;SU Zhanqiang;XIA Jun(College of Animal Medicine,Xinjiang Agricultural University,Urumqi 830000,China;Xinjiang Institute of Animal Health Supervision,Urumqi 830000,China;Veterinary Research Institute of Xinjiang Academy of Animal Sciences/Animal Clinical Medical Research Center of Xinjiang Academy of Animal Husbandry,Urumqi 830000,China;Key Laboratory for the Prevention and Control of Herbivorous Animal Diseases of the Ministry of Agriculture and Rural Affairs,Urumqi 830000,China;College of Animal Science and Technology,Shihezi University,Shihezi,Xinjiang 832061,China)

机构地区：[1]新疆农业大学动物医学学院,乌鲁木齐830000 [2]新疆维吾尔自治区动物卫生监督所,乌鲁木齐830000 [3]新疆畜牧科学院兽医研究所/新疆畜牧科学院动物临床医学研究中心,乌鲁木齐830000 [4]农业农村部草食动物疫病防控重点实验室(省部共建),乌鲁木齐830000 [5]石河子大学动物科技学院,新疆石河子832061

出　　处：《草食家畜》2023年第5期13-17,共5页Grass-Feeding Livestock

基　　金：新疆维吾尔自治区中央引导地方项目“羊多联多价疫苗集成研究与应用”(ZYYD2022B16);新疆维吾尔自治区重大科技专项“新疆畜禽疫病防控体系质量提升工程”(2022273334);新疆维吾尔自治区天山英才青年科技拔尖人才项目“牛羊幼畜群发病防控关键技术研究”(2022196128);新疆维吾尔自治区多胎多羔羊健康养殖与品质提升关键技术研究及示范项目(2021YFD1600701-2);新疆维吾尔自治区重点研发计划项目“肉羊饲料营养、胃肠道调控及废弃物资源化处理关键技术研究”(2022B02056-2);新疆维吾尔自治区现代农业产业体系“昌吉综合实验站站长项目”。

摘　　要：【目的】新疆某牛场的犊牛发生严重腹泻,出现排血便、黄色水样稀便、精神萎靡、食欲不振等症状。为了确定此次发病的病原做了此实验。【方法】无菌采集了20份犊牛粪便样品,从粪便中分离纯化出1株细菌,并对其进行培养特性观察、PCR鉴定、黏附性基因检测、药敏试验和动物致病性试验。【结果】结果显示分离到了一株革兰氏阴性菌,根据形状和大小判断疑似大肠杆菌;PCR鉴定结果显示为大肠杆菌,分离菌株具有F41黏附性基因。该分离菌株对多黏菌素B、阿米卡星、头孢他啶、头孢哌酮等敏感;在致病性试验中,小鼠在15h内陆续死亡,说明该菌株具有一定致病力。【结论】结果表明,此次牛场犊牛腹泻分离的菌株为产肠毒素性大肠杆菌,携带F41毒力基因,对多黏菌素B、阿米卡星等抗生素敏感,具有一定致病力。【Objective】Calves in a cattle farm in Xinjiang showed severe diarrhoea with symptoms such as bloody stools,yellow watery stools,depression,and loss of appetite.The aim of the study was to determine the pathogen of this disease.【Methods】Twenty calf faecal samples were collected aseptically,and one bacteria strain was isolated and purified,and was subjected to culture characteristic observation,PCR identification,adhesion gene detection,drug sensitivity test and animal pathogenicity test.【Results】The results showed that a Gram-negative bacterial strain was isolated,and it was suspected to be Escherichia coli according to its shape and size.The results of PCR showed that it was Escherichia coli,and the isolated strain had F41 adhesion gene.The isolated strain was sensitive to polymyxin B,amikacin,ceftazidime and cefoperazone.In the pathogenicity test,the mice died one after another within 15 hours,which indicated that the strain had certain pathogenicity.【Conclusion】The results showed that the strain isolated this time was enterotoxin-producing Escherichia coli,carring F41 virulence gene,and was sensitive to antibiotics such as polymyxin B and amikacin,and had a certain pathogenicity.

关 键 词：犊牛腹泻 大肠杆菌 药敏试验 F41 

分 类 号：S852.61[农业科学—基础兽医学]