汉麻植物活性物/单宁酸/AgNPs/PAN复合微纳纤维膜的实验设计及抗菌活性研究  

Experimental design and antimicrobial activity of composite micro-nanofiber membranes of hemp plant actives/tannic acid/AgNPs/PAN

作　　者：林冬松 谢晓琪 陈品鸿 李定帆 赵慧 曹庆云[2] 周武艺 LIN Dongsong;XIE Xiaoqi;CHEN Pinhong;LI Dingfan;ZHAO Hui;CAO Qingyun;ZHOU Wuyi(Biomass 3D Printing Research Center,College of Materials and Energy,South China Agricultural University,Guangzhou 510642,China;College of Animal Science,South China Agricultural University,Guangzhou 510642,China)

机构地区：[1]华南农业大学材料与能源学院生物3D打印研究中心,广东广州510642 [2]华南农业大学动物科学学院,广东广州510642

出　　处：《实验技术与管理》2023年第8期27-32,共6页Experimental Technology and Management

基　　金：2022年度广东省本科高校教学质量与教学改革工程项目(粤教高函[2023]4号);华南农业大学2020年校级线上线下混合式课程、线下课程建设项目(华南农教[2020]32号);广州市荔湾区科技项目(202201063);2021年华南农业大学校级教育教学改革项目(JG21131)。

摘　　要：汉麻植物含有丰富的抗菌类活性物,可缓解由抗生素滥用导致的耐药性问题。该文使用高压静电纺丝技术设计了一种复合抗菌微纳米纤维膜,包含汉麻植物活性物质(HPA)、单宁酸(TA)、银纳米颗粒(Ag NPs)和聚丙烯腈(PAN)。实验发现:在PAN质量浓度为0.1kg/L、HPA和TA质量浓度均为0.03kg/L、AgNO3质量浓度为0.01 kg/L、施加电压为17 kV、接收距离为14 cm、供液速率为1 mL/h时,制备条件最佳;在HPA的添加质量浓度为0.03kg/L时微纳纤维的形貌均一,直径均匀,药物负载和热稳定性良好;抗菌测试结果显示,不同HPA质量浓度的微纳纤维膜均能有效抑制大肠杆菌和金黄色葡萄球菌的生长,其中对金黄色葡萄球菌的抗菌作用明显,抗菌率均达95%以上。因此,该实验设计和制备的HPA/TA/Ag/PAN复合纤维膜在抗菌方面具有良好的应用前景。Hemp plant is rich in antibacterial active substances,which can alleviate the problem of drug resistance due to the abuse of antibiotics.A composite antibacterial micro-nanofiber membrane is designed using high-voltage electrospinning technology,containing hemp plant actives(HPA),tannic acid(TA),silver nanoparticles(AgNPs)and polyacrylonitrile(PAN).Experimental results show that the optimal preparation conditions are at a PAN mass concentration of 0.1 kg/L,HPA and TA mass concentrations of 0.03 kg/L,AgNO3 concentration of 0.01 kg/L,an applied voltage of 17 kV,a receiving distance of 14 cm,and a liquid supply rate of 1 mL/h.The micro-nanofiber membranes added with 0.03 kg/L HPA are found to have homogeneous morphology,uniform diameter,good drug loading and thermal stability.The antibacterial experiments show that the micro-nanofiber membranes with different HPA concentrations has good antibacterial effects on both E.coli and S.aureus,among which the antibacterial effects on S.aureus are obvious,with the antibacterial rates reaching over 95%.Therefore,the experimental design and the obtained HPA/TA/Ag/PAN composite fiber membranes have good prospects for antibacterial applications.

关 键 词：高压静电纺丝法 汉麻植物活性物 抗菌微纳纤维膜 聚丙烯腈 

分 类 号：TQ340.64[化学工程—化纤工业]