miR-135a-5p对氟他胺致隐睾小鼠胰岛素样因子3及睾酮表达的影响  

Effects of miR-135a-5p on the expression of insulin-like factor 3 and testosterone in flutamide-induced cryptorchidism mouse

作　　者：杨远贵 安妮妮[1] 陈辉[1] 何国庆[1] 占雄[1] 吴谋东 汪丹 王蔚 彭金普 Yang Yuangui;An Nini;Chen Hui;He Guoqing;Zhan Xiong;Wu Moudong;Wang Dan;Wang Wei;Peng Jinpu(Dept of Pediatric Surgery,Guizhou Provincial People′s Hospital,Guiyang 550002)

机构地区：[1]贵州省人民医院小儿外科,贵阳550002

出　　处：《安徽医科大学学报》2023年第10期1662-1665,共4页Acta Universitatis Medicinalis Anhui

基　　金：贵州省科技计划项目(编号:黔科合基础[2018]1102)。

摘　　要：目的探讨miR-135a-5p对氟他胺导致的隐睾症小鼠睾丸组织中胰岛素样因子3(INSL3)和睾酮表达水平的影响。方法构建氟他胺致隐睾小鼠模型,将实验分为正常对照组、氟他胺组、氟他胺+miR-135a-5p敲降组及氟他胺+miR-135a-5p过表达组。用RT-qPCR法检测小鼠睾丸组织中miR-135a-5p和INSL3 mRNA的表达水平;用Western blot检测INSL3蛋白的表达水平;用ELISA法检测睾酮的表达水平。结果氟他胺致隐睾小鼠睾丸组织中miR-135a-5p、INSL3 mRNA和蛋白以及睾酮的表达水平均显著下调(P<0.05)。敲降miR-135a-5p可下调隐睾睾丸组织中INSL3 mRNA和蛋白以及睾酮的表达水平(P<0.01),而过表达miR-135a-5p则具有相反的结果。结论miR-135a-5p在氟他胺致隐睾小鼠睾丸组织中低表达,过表达miR-135a-5p可恢复INSL3和睾酮的表达水平。Objective To investigate the effect of miR-135a-5p on the expression levels of insulin-like factor 3(INSL3)and testosterone in the testicular tissues of flutamide-induced cryptorchidism mouse.Methods A model of flutamide-induced cryptorchidism in mouse was constructed,and the experiment was divided into normal control group,flutamide group,flutamide+miR-135a-5p knockdown group and flutamide+miR-135a-5p overexpression group.RT-qPCR was used to detect the expression levels of miR-135a-5p and INSL3 mRNA.Western blot was employed to assess the protein expression level of INSL3.ELISA was performed to measure the expression level of testosterone.Results The expression levels of miR-135a-5p,INSL3 mRNA and protein and testosterone were significantly down-regulated in the testis of cryptorchid mice by flutamide(P<0.05).Knockdown of miR-135a-5p could downregulate the expression of INSL3 mRNA,INSL3 protein and testosterone(P<0.01),while overexpression of miR-135a-5p had the opposite result.Conclusion miR-135a-5p decreased in flutamide-induced cryptorchidism mouse testicular tissues,and overexpression of miR-135a-5p could restore the expression levels of INSL3 and testosterone.

关 键 词：隐睾症 氟他胺 miR-135a-5p 胰岛素样因子3 睾酮 

分 类 号：R339.2[医药卫生—人体生理学]