IL-22/STAT3信号通路在慢性间歇低氧致胰岛素抵抗中的作用  

The role of IL-22/STAT3 pathway in insulin resistance caused by intermittent hypoxia

作　　者：刘宏飞 孙婷婷[1] 田稼荟 陈新洁 任丽珏[1] 杜婧[1] 韩芳[2] 魏翠英[1] Liu Hongfei;Sun Tingting;Tian Jiahui;Chen Xinjie;Ren Lijue;Du Jing;Han Fang;Wei Cuiying(Dept of Inner Mongolia University of Science and Technology,The First Affiliated Hospital of Baotou Medical College,Baotou 014010;Sleep Medicine Center,Peking University People′s Hospital,Beijing 100044)

机构地区：[1]内蒙古科技大学包头医学院第一附属医院内分泌科,包头014010 [2]北京大学人民医院睡眠医学中心,北京100044

出　　处：《安徽医科大学学报》2023年第10期1678-1682,共5页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81660020);内蒙古自然科学基金(编号:2019MS08192);内蒙古科技计划项目(编号:2021GG0219)。

摘　　要：目的对照观察大鼠在慢性间歇低氧-复氧条件下,白细胞介素-22(IL-22)、信号传导及转录激活因子3(STAT3)与空腹血糖(FBG)、空腹胰岛素(FINS)的关系,探讨IL-22/STAT3通路在慢性间歇低氧所致胰岛素抵抗中的作用。方法将24只SD大鼠随机分为对照组(NC组)和间歇低氧组(CIH组),每组各12只。NC组置于常氧环境12周,CIH组先予间歇低氧8周,随后恢复常氧饲养至第12周。两组均于基线、第8周、第12周测定FBG、FINS、IL-22、p-STAT3/STAT3水平,并计算胰岛素抵抗指数(HOMA-IR),比较组间差异。结果①两组基线时各观察指标之间差异无统计学意义(P>0.05);8周时,与NC组比较,CIH组FBG、FINS、HOMA-IR水平增高、IL-22水平降低(均P<0.05),p-STAT3/STAT3有降低趋势,但差异无统计学意义;复氧4周时,两组FBG、FINS、HOMA-IR、IL-22水平比较差异无统计学意义(均P>0.05),CIH组p-STAT3/STAT3值较NC组增高(P<0.05)。②Spearman秩相关分析显示,HOMA-IR与IL-22、p-STAT3/STAT3均呈负相关(均P<0.05)。结论慢性间歇低氧能够抑制IL-22/STAT3信号通路的表达;IL-22/STAT3信号通路可能介导了慢性间歇低氧所致的胰岛素抵抗。Objective By observing the changes of interleukin-22(IL-22),signal transduction and transcriptional activator 3(STAT3),fasting blood glucose(FBG)and fasting insulin(FINS)of rats under the circumstance of chronic intermittent hypoxia and reoxygenation,to explore the role of IL-22/STAT3 pathway in insulin resistance induced by chronic intermittent hypoxia.Methods 24 SD rats were randomly divided into control group(NC group)and intermittent hypoxia group(CIH group),with 12 rats in each group.NC group was placed in normoxia environ-ment for 12 weeks,while CIH group was first given intermittent hypoxia for 8 weeks and then resumed normoxia feeding until 12 weeks.FBG,FINS,IL-22 and p-STAT3/STAT3 levels were measured at baseline,week 8 and week 12 in both groups,and insulin resistance index(HOMA-IR)was calculated.The differences between the two groups were compared.Results①There was no significant difference of the observation indexes between the two groups at baseline(P>0.05).At 8 weeks,the levels of FBG,FINS and HOMA-IR in CIH group were higher than those in NC group(P<0.05),and the levels of IL-22 were lower than those in NC group(P<0.05).p-STAT3/STAT3 showed a decreasing trend,but not statistically significant.At 4 weeks of reoxygenation,there were no significant differences in FBG,FINS,HOMA-IR and IL-22 levels between the two groups(P>0.05).p-STAT3/STAT3 in CIH group was significantly higher than that in NC group(P<0.05).②Spearman rank correlation analysis showed that HOMA-IR was negatively correlated with IL-22 and p-STAT3/STAT3(all P<0.05).Conclusion Chronic intermittent hypoxia can inhibit the expression of IL-22/STAT3 signaling pathway,IL-22/STAT3 signaling pathway may mediate insulin resistance induced by chronic intermittent hypoxia.

关 键 词：间歇低氧 胰岛素抵抗 白细胞介素-22 信号传导及转录激活因子3 

分 类 号：R587.1[医药卫生—内分泌]