Combined application of organic manure and chemical fertilizers stabilizes soil N-cycling microflora  

作　　者：Ruibo Sun Daozhong Wang Zhibin Guo Keke Hua Xisheng Guo Yan Chen Binbin Liu Haiyan Chu 

机构地区：[1]State Key Laboratory of Soil and Sustainable Agriculture,Institute of Soil Science,Chinese Academy of Sciences,71 East Beijing Road,Nanjing 210008,China [2]Anhui Province Key Laboratory of Farmland Ecological Conservation and Pollution Prevention,Key Laboratory of JiangHuai Arable Land Resources Protectionand Eco-Restoration,College of Resources and Environment,Anhui Agricultural University,130 Changjiangxilu,Hefei 230036,China [3]Soil and Fertilizer Research Institute,Anhui Academy of Agricultural Sciences,40 South Nongke Road,Hefei 230031,China [4]Key Laboratory of Agricultural Water Resources,Hebei Key Laboratory of Soil Ecology,Center for Agricultural Resources Research,Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,286 Huaizhong Road,Shijiazhuang 050021,China

出　　处：《Soil Ecology Letters》2023年第3期5-11,共7页土壤生态学快报（英文）

基　　金：This work was supported by the Strategic Priority Research Program of the Chinese Academy of Sciences(Grant No.XDB40020204);the National Key R&D Program of China(Grant No.2021YFF1000403).

摘　　要：Straw and manure are widely applied to agricultural systems,and greatly shape soil N-cycling microflora.However,we still lack a comprehensive understanding of how these organic materials structure soil N-cycling microbial communities.In this study,metagenomic analysis was performed to investigate the compositional variation in N-cycling microbial communities in a 30-year long-term experiment under five fertilization regimes:no fertilization(Control),chemical fertilization only(NPK),and NPK with wheat straw(NPK+HS),pig manure(NPK+PM),and cow manure(NPK+CM).Long-term NPK application differentially changed N-cycling gene abundance and greatly altered N-cycling microbial community structure.NPK+HS resulted in a similar pattern to NPK in terms of gene abundance and community structure.However,NPK+PM and NPK+CM significantly increased most genes and resulted in a community similar to that of the Control.Further analysis revealed that serious soil acidification caused by long-term NPK fertilization was a major factor for the variation in N-cycling microbial communities.The addition of alkaline manure,rather than wheat straw,stabilized the N-cycling microbial community structure presumably by alleviating soil acidification.These results revealed the strong impact of soil acidification on microbial N-cycling communities and illustrated the possibility of resolving nitrogen-related environmental problems by manipulating pH in acidified agricultural soils.

关 键 词：microbial nitrogen cycle METAGENOMICS long-term fertilization soil acidification MANURE 

分 类 号：S15[农业科学—土壤学]