Positive and negative effects of graphite flake and monolayer graphene oxide templates on protein crystallization  

作　　者：Zhichun Lin Wenqing Tian Yang Su Vikash Kumar Yadav Huaiyu Yang 

机构地区：[1]Department of Chemical Engineering,Loughborough University,Loughborough,LE113TU,UK [2]Institute of Materials Research,Tsinghua Shenzhen International Graduate School,Tsinghua University,Shenzhen,518055,China [3]Human Sciences Research Centre,University of Derby,DE221GB,UK

出　　处：《Particuology》2023年第8期27-34,共8页颗粒学报（英文版）

基　　金：grateful to the UK EPSRC(Engineering and Physical Sciences Research Council)for support(EP/T005378/1).

摘　　要：Heterogeneous template-induced nucleation is a promising way to regulate protein crystallization events and could be employed for purification processes and crystallographic studies.Protein crystallization process with graphite and graphene oxide,as heterogeneous templates,were investigated.More than 640 hanging drops with different concentrations of Lysozyme(30,50,70,100 mg/mL)and NaCl(0.7,0.9,1.1,1.3,1.5 M)were crystallised at 4 ℃ with or without graphite/graphene oxide templates.The induction times and crystallization process were observed under the microscope.The lysozyme in the solutions with graphite flakes nucleated faster under all the conditions than the lysozyme with equal experimental conditions without templates.The crystals preferred to grow around the edge of graphite flakes than on the flat surfaces.In the droplets with monolayer graphene oxide,more crystals appeared around gra-phene oxide particles,and the faster or slower nucleation processes with templates were dependent on the lysozyme and NaCl concentrations.Graphene oxide templates strongly inhibited nucleation at high lysozyme concentrations but promoted nucleation at low lysozyme concentrations.Both heterogeneous templates changed the crystal morphology and the crystallization kinetics.More crystals were observed in the solution with graphite templatesthan with graphene oxide templates and without any template.

关 键 词：GRAPHITE Graphene oxide Protein crystallization ADSORPTION Heterogeneous nucleation Template technology 

分 类 号：TQ127.11[化学工程—无机化工]