Preparation of slow-release regulated Rs-198 bilayer microcapsules and application of its lyophilized bacterial inoculant on Capsicum annuum L.under salt stress  

作　　者：Shuangxi Zhu Yanhui He Jiawei Dong Yujia Dong Chun Li Zhansheng Wu Yimeng Lu 

机构地区：[1]Xi'an Key Laboratory of Textile Chemical Engineering Auxiliaries,School of Environmental and Chemical Engineering,Xi'an Polytechnic University,Xi'an,710048,China [2]Department of Chemical Engineering,Tsinghua University,Beijing,100084,China

出　　处：《Particuology》2023年第8期54-63,共10页颗粒学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(grant No.U1803332,22278325);Xi'an Science and Technology Plan Project Agricultural Technology R&D Project(grant No.21NYYF0030,22NYYF037);the Scientific Research Plan for Local Special Service of Shaanxi Provincial Education Department(grant No.20JC014);Preferential Funding Projects for Scientific and Technological Activities of Overseas Scholar(grant No.2020018);Key Research and Development Program of Xianyang City(grant No.S2021ZDYF-NY-0024)。

摘　　要：The survival adaptation of bacteria in saline soil is poor.The bilayer microcapsules were prepared by secondary embedding of monolayer sodium alginate(NaAlg)-bentonite(Bent)-sodium carboxy-methylcellulose(CMC)microcapsules wrapped with plant growth promoting rhizobacteria(PGPR)Pseudomonas putida Rs-198 by chitosan solution to promote the synergistic effect of bilayer microen-capsulation and PGPR.The characterization of the Rs-198 bilayer microcapsules showed that the amino and carboxyl groups were cross-linked and a thin layer of chitosan was formed on the outside of the microcapsule.The bilayer microcapsule(Ch-d)with a chitosan concentration of 0.8 wt%and pH 6 showed a slow release of bacteria with a maximum release of 6.06 × 10^(9) cfu/g on the 7th day.The viable bacteria of Ch-d increased by 4.42%after 60 days of storage compared with monolayer microcapsules.The 0.9 wt%L-cysteine,10 wt%glycerinum,10 wt%trehalose and 12 wt%soluble starch were added as bacterial protective agents during the process of preparing the Ch-d lyophilized bacterial inoculant(Ch-d LBI).Pot experiments showed that Ch-d LBI exhibited better growth promotion of Capsicum annuum L.under salt stress.Therefore,the bilayer microcapsule as slow-release bacterial inoculant is a potential alternative for sustainable agriculture.

关 键 词：Pseudomonas putida Rs-198 Bilayer microcapsules Chitosan solution Lyophilized bacterial inoculant 

分 类 号：S64[农业科学—蔬菜学]