基于太赫兹超材料的牛血清白蛋白传感器研究  

Research of Bovine Serum Albumin Sensor Based on Terahertz Metamaterials

作　　者：郑卓锐 钟慧 聂勇潇 林婷 方依霏 宋立伟[1,4] 田野 Zheng Zhuorui;Zhong Hui;Nie Yongxiao;Lin Ting;Fang Yifei;Song Liwei;Tian Ye(State Key Laboratory of High Field Laser Physics,Shanghai Institute of Optics and Fine Mechanics,Chinese Academy of Sciences,Shanghai 201800,China;School of Optical and Electronic Information,Huazhong University of Science and Technology,Wuhan 430074,Hubei,China;College of Optical-Electrical and Computer Engineering,University of Shanghai for Science and Technology,Shanghai 200093,China;Center of Materials Science and Optoelectronics Engineering,University of Chinese Academy of Sciences,Beijing 100049,China)

机构地区：[1]中国科学院上海光学精密机械研究所强场激光物理国家重点实验室,上海201800 [2]华中科技大学光学与电子信息学院,湖北武汉430074 [3]上海理工大学光电信息与计算机工程学院,上海200093 [4]中国科学院大学材料与光电研究中心,北京100049

出　　处：《中国激光》2023年第17期200-207,共8页Chinese Journal of Lasers

基　　金：国家自然科学基金(62105346);科技部重点研发专项(2022YFA1604400);基础研究特区计划(JCYJ-SHFY-2021-002);中国科学院基础前沿科学研究计划(ZDBS-LY-SLH018);中国科学院青年促进会项目、强场激光物理国家重点实验室开放课题。

摘　　要：太赫兹波具有非电离、对非极性物质穿透性高、对氢键等弱共振敏感等特性,是生物、材料、化学等领域的重要研究对象。蛋白质、糖类等生物大分子的转动频率和基团的振动频率以及分子之间弱相互作用的特征频率恰好处于太赫兹频段,这赋予了太赫兹光谱技术在生物医学领域中极大的发展潜力。然而,由于待测物尺寸一般小于太赫兹波长(0.03~3.00 mm),微量的待测物难以引起谱线的改变,太赫兹光谱检测灵敏度较低。能够灵活操控电磁波特性的超材料为解决上述问题提供了新的思路,通过设计不同的结构及参数,能够得到谐振频率位于太赫兹波段的超材料,微量的待测物即可引起谱线的明显变化。基于共振型太赫兹超材料构建了牛血清白蛋白(BSA)传感器,实验结果表明,当BSA溶液的体积质量为2.0~8.0 mg/mL时,传感器共振频率偏移量与溶液浓度呈线性关系,传感器的最低浓度检测限为0.3 mg/mL。Objective Terahertz waves are electromagnetic waves with frequencies of 0.1‒10.0 THz.They have the characteristics of wide bandwidth,strong penetration,and low photon energy.Notably,the energy levels of the terahertz spectrum correspond to the rotational and vibrational energy levels of several biological macromolecules.Therefore,the terahertz spectroscopy technology can be used to study the properties of biomolecules,such as their molecular structures and their molecular interactions with the surrounding environment.Because the wavelength of a terahertz wave(0.03‒3.00 mm)is not in the same order of magnitude as the characteristic size of common biological macromolecules,it is difficult to produce sufficient interaction between the trace levels of biological macromolecules contained in the sample and the terahertz wave,and the weak change in the terahertz spectral line is difficult to capture.Metamaterial is an artificial material whose electromagnetic properties can be manipulated artificially.By combining terahertz spectroscopy technology with metamaterials,the small disturbance to the metamaterial caused by trace-level objects can result in significant changes in the spectra of metamaterials,and thus make high-sensitivity detection of biomacromolecules possible.In this study,a resonant terahertz metamaterial is used to enhance the interaction between terahertz wave and the determinand,and a bovine serum albumin(BSA)solution is selected as the analyte.An efficient BSA sensor is constructed using terahertz spectroscopy technology.The relationship between the concentration of the BSA solution and the resonance frequency offset of the sensor is analyzed,and the limit of detection of the sensor is examined.Methods To achieve rapid and highly sensitive detection of BSA solution,the unit structure designed by Sengupta et al.is adopted.Because of the C4 symmetry of the structure,the metamaterial-based sensor is insensitive to polarization.The electromagnetic properties of the metamaterial are simulated by full-wav

关 键 词：光谱学 太赫兹 超材料 牛血清白蛋白 传感器 

分 类 号：O436[机械工程—光学工程]