仙茅苷对七氟醚麻醉老年大鼠术后认知功能的影响及其抗氧化和抗凋亡作用  

Effects of curculigoside on cognitive function and its antioxidant and anti-apoptotic effects in senile rats after sevoflurane anesthesia

作　　者：王菲菲 王海英 吴智宏 陈冬 Wang Feifei;Wang Haiying;Wu Zhihong;Chen Dong(Department of Anesthesiology,North Branch of Qingdao Central Hospital(Qingdao Chest Hospital),Qingdao 266034,China;Qingdao Central Medical Group,the Operating Room,Qingdao 266000,China;Department of Anesthesiology,Beijing Electric Power Hospital of State Grid Corporation,Beijing 100071,China)

机构地区：[1]青岛市中心医院北部院区(青岛市胸科医院)麻醉科,青岛266034 [2]青岛市中心医疗集团手术室,青岛266000 [3]国家电网公司北京电力医院麻醉科,北京100071

出　　处：《国际麻醉学与复苏杂志》2023年第9期904-908,共5页International Journal of Anesthesiology and Resuscitation

摘　　要：目的探究仙茅苷对七氟醚麻醉老年大鼠术后认知功能的影响及其抗氧化和抗凋亡作用。方法 30只大鼠按照随机数字表法分为两组(每组15只):围手术期神经认知障碍(perioperative neurocognitive disorders, PND)组和PND+仙茅苷组。两组大鼠根据参考文献建立PND模型, 吸入七氟醚麻醉后进行腹腔探查。PND+仙茅苷组大鼠术后以仙茅苷(50 mg/kg)灌胃, 每日1次, 连续7 d;PND组大鼠灌胃等量生理盐水。记录大鼠水迷宫实验目标象限停留时间和穿越平台次数, H-E染色检测海马组织损伤情况, ELISA法检测超氧化物歧化酶(superoxide dismutase, SOD)和丙二醛(malondialdehyde, MDA)水平, TUNEL染色检测海马神经元凋亡指数, Western blot法检测蛋白激酶B(protein kinase B, Akt)磷酸化水平。结果与PND组比较, PND+仙茅苷组大鼠目标象限停留时间、穿越平台次数明显增加(P<0.05), 磷酸化Akt(phosphorylated-Akt, p-Akt)、Akt升高(P<0.05), SOD水平、Akt磷酸化水平明显升高(P<0.05), MDA和凋亡指数明显降低(P<0.05), 海马组织损伤减轻。结论仙茅苷具有抑制氧化应激和海马神经元凋亡保护作用, 改善老年PND大鼠的认知功能。Objective To investigate the effects of curculigoside on postoperative cognitive function and its antioxidant and anti-apoptotic effects in elderly rats.Methods Thirty rats were divided into two groups(15 in each group)according to the random number table method:perioperative neurocognitive disorders(PND)group and PND+curculigoside group.PND model was established according to the references,sevoflurane was inhaled into rats in PND group and PND+curculigoside group for abdominal exploration.Rats in PND+curculigoside group were given curculigoside(50 mg/kg)intragastric administration after operation,once a day for consecutive 7 days;PND group was given the same amount of normal saline.The target quadrant residence time and platform crossing times of the water maze were recorded,hematoxylin-eosin(H-E)staining was used to detect hippocampal injury,and enzyme-linked immunosorbent assay(ELISA)was used to detect superoxide dismutase(SOD)and malondialdehyde(MDA)levels,the apoptosis index of hippocampal neurons was detected by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling(TUNEL)staining and the phosphorylation level of protein kinase B(Akt)was detected by Western blot.Results Compared with PND group,the target quadrant residence time,the number of platform crossing were significantly increased(P<0.05),hippocampal tissue damage was reduced,phosphorylated-Akt(p-Akt)and Akt were increased(P<0.05),SOD level and Akt phosphorylation level were significantly increased(P<0.05),MDA and apoptosis index were significantly decreased(P<0.05)in PND+curculigoside group.Conclusions Curculigoside can inhibit oxidative stress and protect hippocampal neurons from apoptosis,and improve the cognitive function of elderly PND rats.

关 键 词：仙茅苷 七氟醚 麻醉 认知功能 抗氧化 抗凋亡 

分 类 号：R965[医药卫生—药理学]