用于核酸现场检测的直轴型多通道光学检测系统  

A Straight-Axis Multi-Channel Optical Detection System for Nucleic Acid On-Site Detection

作　　者：奚邦朝 杨佳羽 黄绍磊 戴皓正 曾俊添 张东旭 Xi Bangchao;Yang Jiayu;Huang Shaolei;Dai Haozheng;Zeng Juntian;Zhang Dongxu(School of Public Health,Xiamen University,Xiamen 361102,Fujian,China;State Key Laboratory of Molecular Vaccinology and Molecular Diagnostic(Xiamen University),Xiamen 361102,Fujian,China;National Institute of Diagnostics and Vaccine Development in Infection Diseases(Xiamen University),Xiamen 361102,Fujian,China;State Drug Administration Key Laboratory of Infectious Disease Detection Technology Research and Evaluation,Xiamen 361102,Fujian,China)

机构地区：[1]厦门大学公共卫生学院,福建厦门361102 [2]分子疫苗学和分子诊断学国家重点实验室(厦门大学),福建厦门361102 [3]国家传染病诊断试剂与疫苗工程技术研究中心(厦门大学),福建厦门361102 [4]国家药品监督管理局传染性疾病检测技术研究与评价重点实验室,福建厦门361102

出　　处：《激光与光电子学进展》2023年第17期325-333,共9页Laser & Optoelectronics Progress

基　　金：国家自然科学基金青年基金(62003284);厦门大学校长基金(20720210089)。

摘　　要：随着医学检验对核酸检测技术要求的不断提高,现场检测(POCT)已经成为该领域的研究重点和难点。为了规避目前使用的POCT核酸检测仪器的缺点,构建了一种直轴型多通道光学检测系统,兼顾实现了多通道核酸实时荧光检测与即地即检。首先,基于正交式几何关系搭建了光路;然后,设计了一种新的直轴型多通道集成与切换装置以实现多个荧光检测通道的实时切换;最后,通过仿真验证了光路设计的有效性,并将本系统集成于自主设计的聚合酶链式反应装置上,以新型冠状病毒标准品进行了实时荧光检测实验。实验结果显示:本系统的变异系数最小可达0.02%;通道分辨率最低可达0.49μg/mL,且没有通道串扰。同时,拥有良好的现场检测能力,可以配合仪器完成“样本进、结果出”的现场检测,为核酸POCT的多通道实时荧光检测提供了一种新思路。With the continuous demand for technological advances in nucleic acid detection for use in medical testing,pointof-care testing(POCT)has emerged as a research focus,presenting various challenges to overcome.To circumvent the disadvantages of POCT nucleic acid detection instruments that are currently in use,a straight-axis multi-channel optical detection system is proposed and designed,which facilitates both real-time,multi-channel as well as instant detection of nucleic acids.First,an optical path is built based on an orthogonal optical path design.Second,a new straight-axis multichannel integration and switching device is designed to enable real-time switching between multiple fluorescence detection channels.Finally,the effectiveness of the optical path design is verified by simulation;this system is integrated into a selfdesigned polymerase chain reaction thermocycler,and a real-time fluorescence detection experiment is conducted using a new coronavirus standard.This system reaches a minimum coefficient of variation value of 0.02%.The minimum channel resolution is 0.49 mg/mL,and no channel crosstalk is observed.The system demonstrates good on-site detection capabilities,and functions cooperatively with the instrument to complete on-site detection from“sample in”to“result out”,offering a new perspective for POCT using multi-channel real-time fluorescence detection of nucleic acids.

关 键 词：医用光学与生物技术 核酸现场检测 光路设计 多通道光学检测 

分 类 号：O439[机械工程—光学工程]