肉苁蓉治疗脊髓损伤分子机制分析  

Molecular Mechanism Analysis of Cistanche Herba in Treating Spinal Cord Injury

作　　者：周亚净[1] 何润之[2] 程芳[3] 刘建敏[4] Zhou Yajing;He Runzhi;Cheng Fang;Liu Jianmin(Department of Anesthesiology,Xingtai People's Hospital,Xingtai 054001,China;Third Department of Neurosurgey,Xingtai People's Hospital,Xingtai 054001,China;Department of Dermatology,Xingtai People's Hospital,Xingtai 054001,China;Department of Hand and Foot Surgery,Xingtai People's Hospital,Xingtai 054001,China)

机构地区：[1]邢台市人民医院麻醉科,河北邢台054001 [2]邢台市人民医院神经外三科,河北邢台054001 [3]邢台市人民医院皮肤科,河北邢台054001 [4]邢台市人民医院手足外科,河北邢台054001

出　　处：《南开大学学报（自然科学版）》2023年第3期39-45,共7页Acta Scientiarum Naturalium Universitatis Nankaiensis

基　　金：河北省青年拔尖人才支持项目。

摘　　要：脊髓损伤(SCI)是一种致命的中枢神经系统创伤,目前尚无有效修复手段.肉苁蓉提取物可以治疗脊髓损伤,但是,作用机制仍然不清楚.运用网络药理学和分子对接技术探讨肉苁蓉治疗SCI的分子机制.肉苁蓉的活性成分和靶点由中药系统药理学数据库和分析平台(TCMSP)预测.SCI相关的靶点从Gene Cards、Drug Bank、Pharm Gkb和OMIM数据库中获得,交集靶点由Venny2.1.0获得.通过String数据库和Cytoscape软件绘制了蛋白质相互作用(PPI)网络.接下来,利用Bioconductor平台进行KEGG通路分析.使用Autodock Vina进行分子对接.根据筛选标准,从肉苁蓉中得到6个活性成分和85个潜在靶基因.通过在线数据库,得到6737个SCI相关靶点和肉苁蓉与SCI的74个交集靶点.在活性成分靶点网络中,β-谷甾醇、槲皮素和花生四烯酸是重要的活性成分.在PPI网络中,HIF1A、FOS、AR、RELA、EGFR和CCD1等是核心靶点.KEGG分析表明肉苁蓉治疗SCI的主要途径涉及PI3K-Akt、TNF和MAPK等信号通路.在分子对接中,肉苁蓉的活性化合物槲皮素与核心靶点具有良好的的亲和力.最后,体外实验验证表明,肉苁蓉对SCI的治疗可能主要是通过其活性成分槲皮素抑制炎症和氧化反应,从而改善SCI微环境.为进一步肉苁蓉治疗SCI的临床应用提供了新思路.Spinal cord injury(SCI)is a fatal central nervous system injury,and there is currently no effective repair method.The extract of cistanche herba can treat spinal cord injury,but the mechanism of action is still unclear.The network pharmacology and molecular docking techniques were used to explore the molecular mechanism of cistanche herba in treating SCI.The active ingredients and targets of cistanche herba were predicted by TCMSP.SCI related targets were obtained from GeneCards,DrugBank,PharmaGkb,and OMIM databases,while intersection targets were obtained from Venny2.1.0.A protein interaction(PPI)network was drawn using a String database and Cytoscape software.Next,the Bioconductor platform was analysed for KEGG pathways.According to the screening criteria,6 active ingredients and 85 potential target genes were obtained from cistanche herba.Through the online database,6737 SCI related targets were identified,and 74 intersection targets of cistanche herba and SCI were obtained.In the target network of active ingredients,β-Valsitosterol,quercetin,and sulazilide are important active ingredients.In the PPI network,HIF1A,FOS,AR,RELA,EGFR,and CCD1 are the core objectives.KEGG analysis showed that the main pathway of cistanche herba in treating SCI involves signaling pathways such as PI3K-Akt,TNF-1,and MAPK.In molecular docking,the active compound of cistanche herba had good affinity for core targets.Finally,in vitro experimental verification shows that cistanche herba may improve the microenvironment of SCI by inhibiting inflammation and oxidation through its active ingredient quercetin.This provides a new approach for the clinical application of cistanche herba in the treatment of SCI.

关 键 词：脊髓损伤 肉苁蓉 分子机制 网络药理 炎症反应 氧化反应 

分 类 号：R68[医药卫生—骨科学]