妊娠期糖尿病孕妇单核细胞趋化蛋白-1、增殖细胞核抗原和核因子-κBp65表达水平及其与妊娠结局的关系  

The relationship between the expression levels of monocyte chemotactic protein-1,proliferating cell nuclear antigen,nuclear factor-κBp65 and pregnancy outcome in pregnant women with gestational diabetesmellitus

作　　者：王艳楠 罗晶 曲冬颖 肖育红 梁琳琅 Wang Yannan;Luo Jing;Qu Dongying;Xiao Yuhong;Liang Linlang(Department of Endocrinology,PLA Northern Theater General Hospital,Shenyang 110000,China)

机构地区：[1]中国人民解放军北部战区总医院内分泌科,沈阳110000

出　　处：《中国医师进修杂志》2023年第9期816-821,共6页Chinese Journal of Postgraduates of Medicine

基　　金：辽宁省科学技术计划项目(2021JH2/10300095)。

摘　　要：目的:探究妊娠期糖尿病(GDM)孕妇胎盘组织单核细胞趋化蛋白-1(MCP-1)、核因子-κBp65(NF-κBp65)和增殖细胞核抗原(PCNA)表达水平及其与妊娠结局的相关性。方法:回顾性分析中国人民解放军北部战区总医院2020年5月至2021年12月124例GDM孕妇的临床资料。其中,62例孕妇愿意接受治疗(治疗组),62例孕妇不愿意接受治疗(未治疗组)。另外选取同期80例健康孕妇作为健康对照组。记录自然顺产率、新生儿Apgar评分及巨大儿、新生儿低血糖和新生儿高胆红素血症发生率。采用免疫组织化学法检测胎盘组织MCP-1、NF-κBp65和PCNA表达情况。结果:未治疗组自然顺产率明显低于治疗组和健康对照组[24.19%(15/62)比75.81%(47/62)和88.75%(71/80)],治疗组自然顺产率明显低于健康对照组,差异有统计学意义( P<0.05)。未治疗组Apgar评分明显低于治疗组和健康对照组[(8.45 ± 2.02)分比(9.46 ± 2.59)和(9.71 ± 3.21)分],巨大儿、新生儿低血糖和新生儿高胆红素血症发生率明显高于治疗组和健康对照组[35.48%(22/62)比11.29%(7/62)和3.75%(3/80)、29.03%(18/62)比8.06%(5/62)和2.50%(2/80)、24.19%(15/62)比9.68%(6/62)和2.50%(2/80)],差异有统计学意义( P<0.05);治疗组与健康对照组各指标比较差异无统计学意义( P>0.05)。未治疗组MCP-1、NF-κBp65和PCNA阳性表达率明显高于治疗组和健康对照组[72.58%(45/62)比25.81%(16/62)和12.50%(10/80)、69.35%(43/62)比27.43%(17/62)和13.75%(11/80)、69.35%(43/62)比24.19%(15/62)和11.25%(9/80)],治疗组明显高于健康对照组,差异有统计学意义( P<0.05)。 结论:GDM孕妇胎盘中MCP-1、NF-κBp65和PCNA表达与不良妊娠结局有关;GDM孕妇给予积极治疗后三项指标阳性率明显降低,改善预后。Objective To investigate the placenta tissue expression levels of monocyte chemotactic protein-1(MCP-1),nuclear factor-κBp65(NF-κBp65)and proliferating cell nuclear antigen(PCNA)in pregnant women with gestational diabetes mellitus(GDM),and to analyze their correlation with pregnancy outcomes.Methods The clinical data of 124 pregnant women with GDM from May 2020 to December 2021 in PLA Northern Theater General Hospital were retrospectively analyzed.Among them,62 pregnant women were willing to receive treatment(treatment group),while 62 pregnant women were unwilling to receive treatment(untreated group).In addition,80 healthy pregnant women in the same period were selected as the healthy control group.The natural birth rate,neonatal Apgar score and the incidences of macrosomia,neonatal hypoglycemia,neonatal hyperbilirubinemia were record.The placenta tissue expression levels of MCP-1,NF-κBp65 and PCNA were detected by immunohistochemical.Results The natural birth rate in untreated group was significantly lower than that in treatment group and healthy control group:24.19%(15/62)vs.75.81%(47/62)and 88.75%(71/80),the natural birth rate in treatment group was significantly lower than that in healthy control group,and there was statistical difference(P<0.05).The Apgar score in untreated group was significantly lower than that in treatment group and healthy control group:(8.45±2.02)scores vs.(9.46±2.59)and(9.71±3.21)scores,the incidences of macrosomia,neonatal hypoglycemia and neonatal hyperbilirubinemia were significantly higher than those in treatment group and healthy control group:35.48%(22/62)vs.11.29%(7/62)and 3.75%(3/80),29.03%(18/62)vs.8.06%(5/62)and 2.50%(2/80),24.19%(15/62)vs.9.68%(6/62)and 2.50%(2/80),and there were statistical differences(P<0.05);there were no statistical difference in the indexes treatment group and healthy control group(P>0.05).The positive expression rates of MCP-1,NF-κBp65 and PCNA in untreated group were significantly higher than those in treatment group and healthy control group:

关 键 词：糖尿病 妊娠 妊娠结局 增殖细胞核抗原 单核细胞趋化蛋白-1 核因子-ΚBP65 

分 类 号：R714.256[医药卫生—妇产科学]