大蒜AsMKK5基因克隆及其在渗透胁迫下的表达分析  

Cloning of AsMKK5 gene in garlic and expression analysis under osmoticstress

作　　者：王广龙 杨舒婷 胡珍珠 周成玲 陈伯清 熊爱生[3] WANG Guanglong;YANG Shuting;HU Zhenzhu;ZHOU Chengling;CHEN Boqing;XIONG Aisheng(School of Life Science and Food Engineering,Huaiyin Institute of Technology,Huaian,Jiangsu 223003,China;Faculty of Applied Technology,Huaiyin Institute of Technology,Huaian,Jiangsu 223003,China;College of Horticulture,Nanjing Agricultural University,State Key Laboratory of Crop Genetics and Germplasm Enhancement,Key Laboratory of Biology and Germplasm Enhancement of Horticultural Crops in East China,Ministry of Agriculture and Rural Affairs,Nanjing 210095,China)

机构地区：[1]淮阴工学院生命科学与食品工程学院,江苏淮安223003 [2]淮阴工学院应用技术学院,江苏淮安223003 [3]南京农业大学园艺学院,作物遗传与种质创新国家重点实验室,农业农村部华东地区园艺作物生物学与种质创制重点实验室,南京210095

出　　处：《植物生理学报》2023年第9期1811-1818,共8页Plant Physiology Journal

基　　金：淮阴工学院博士科研启动基金(Z301B16531);教育部新世纪优秀人才支持计划项目(NCET-11-0670);江苏省自然科学基金杰出青年基金(BK20130027)。

摘　　要：MAPKK是MAPK联级反应中的关键组分,在植物生长发育以及响应环境变化等过程有着重要作用。为了解大蒜MAPKK基因的序列特征及其在渗透胁迫下的作用,利用RT-PCR从大蒜中得到AsMKK5基因,采用DNAMAN和MEGA 5等进行序列分析,并对其在盐胁迫和模拟干旱胁迫下的表达特征进行了鉴定。结果显示,大蒜AsMKK5基因全长978 bp,编码325个氨基酸。在进化关系上,AsMKK5与拟南芥AtMKK4和AtMKK5最为接近。实时荧光定量PCR显示,盐胁迫和干旱胁迫均能诱导AsMKK5基因的表达,说明其可能在大蒜植株响应渗透胁迫的过程中起着重要作用。本研究克隆的AsMKK5基因为大蒜等作物抗逆育种提供了新的基因资源。MAPKK is a key component of MAPK cascade reaction,playing an important role in plant growth and development and responses to environmental changes.In order to understand the sequence charac-teristics of garlic(Allium sativum)MAPKK genes and their roles under osmotic stress,AsMKK5 gene was obtained from garlic by RT-PCR.DNAMAN and MEGA 5 were used for sequence analysis,and its expres-sion characteristics under salt stress and simulated drought stress were identified.The results showed that the total length of garlic AsMKK5 gene was 978 bp,encoding 325 amino acids.In terms of evolutionary relationship,AsMKK5 is closest to Arabidopsis AtMKK4 and AtMKK5.Quantitative real-time fluorescence PCR showed that both salt stress and drought stress could induce the expression of AsMKK5 gene,indicating that it might play an important role in the response of garlic plants to osmotic stresses.The AsMKK5 gene cloned in this study provides new genetic resources for stress resistance breeding of garlic and other crops.

关 键 词：大蒜 AsMKK5基因 渗透胁迫 基因克隆 表达分析 

分 类 号：S633.4[农业科学—蔬菜学]