LED诱导荧光的土壤多环芳烃快速检测方法  

Rapid Detection of Polycyclic Aromatic Hydrocarbons in Soil Using LED-Induced Fluorescence Spectroscopy

作　　者：刘梁晨 杨瑞芳 赵南京[2,3] 石高勇 杨金强 黄朋 殷高方 方丽 刘建国[2,3] 刘文清 Liu Liangchen;Yang Ruifang;Zhao Nanjing;Shi Gaoyong;Yang Jinqiang;Huang Peng;Yin Gaofang;Fang Li;Liu Jianguo;Liu Wenqing(School of Environmental Science and Optoelectronic Technology,University of Science and Technology of China,Hefei 230026,Anhui,China;Key Laboratory of Environmental Optics and Technology,Anhui Institute of Optics and Fine Mechanics,Hefei Institutes of Physical Science,Chinese Academy of Sciences,Hefei 230031,Anhui,China;Key Laboratory of Optical Monitoring Technology for Environment of Anhui Province,Hefei 230031,Anhui,China;School of Biology,Food and Environment,Hefei University,Hefei 230601,Anhui,China)

机构地区：[1]中国科学技术大学环境科学与光电技术学院,安徽合肥230026 [2]中国科学院合肥物质科学研究院安徽光学精密机械研究所中国科学院环境光学与技术重点实验室,安徽合肥230031 [3]安徽省环境光学监测技术重点实验室,安徽合肥230031 [4]合肥学院生物食品与环境学院,安徽合肥230601

出　　处：《光学学报》2023年第18期294-304,共11页Acta Optica Sinica

基　　金：国家重点研发计划子课题(2020YFC1807204-1);中国科学院科技服务网络计划(STS计划)区域重点项目课题(KFJ-STS-QYZD-2021-04-001-4);安徽省重点研究和开发计划(201904a07020089);土壤典型重金属原位检测技术研究(210914002)。

摘　　要：土壤作为多环芳烃类有机污染物的主要环境归宿,使用传统色谱和荧光光谱分析方法对残留在其中的多环芳烃进行检测常存在局限性,难以满足动态快速监测土壤污染状况的实际需求。为了实现对土壤多环芳烃的快速检测,设计搭建255nm紫外LED诱导荧光光谱检测系统,以䓛、荧蒽、菲和芘4种多环芳烃为研究对象,探讨了不同土壤类型中多环芳烃的LED诱导荧光特性,验证了LED诱导荧光快速检测方法应用于土壤多环芳烃污染物检测的可行性。实验结果表明,在一定的浓度范围内,标准黄土、高岭土中多环芳烃在特征荧光峰处的荧光强度与浓度均呈现出良好的线性相关性(R^(2)>0.98)。在对受多环芳烃污染的实际土样进行检测时,通过建立定点波长浓度反演模型来实现对多环芳烃浓度的定量分析,测试集浓度预测的相对误差基本在理想范围内,对不同多环芳烃土样的平均相对误差最大不超过15.5%。Objective Rapid industrialization in China has gradually led to long-term accumulated soil environmental problems.In addition,pollution caused by industrial and agricultural production processes constantly deteriorates soil environmental quality.Polycyclic aromatic hydrocarbons(PAHs)are a class of persistent organic pollutants that are mainly derived from man-made pollution and migrate globally with biogeochemical cycles.Residual PAHs in soil have a profound impact on environmental quality and human health.Therefore,it is of great practical significance to monitor organic pollutants in soil and timely grasp the pollution situation of the regional topsoil.At present,PAHs are detected using field sampling and laboratory instrument analysis.Trace analysis methods based on chromatographic separation have the advantages of low detection limit and high accuracy,but they usually require complex sample pretreatment,complicated operation,and long detection cycle.Thus,studies have developed a light-emitting diode(LED)-induced fluorescence methods using newlyinvented luminescent materials and advanced production technologies.An LED can make up for the deficiencies of traditional excitation light sources,facilitating the production of small instruments.In this paper,the feasibility of using an LED-induced fluorescence spectroscopy technology for the rapid determination of PAHs in soil is discussed based on the fluorescence detection system of UV LED array excitation and a theoretical basis is provided for the application of LEDinduced fluorescence in the rapid,accurate,and real-time detection of soil organic pollutants.Methods In this experiment,four types of PAHs were selected as the research objects.Two different types of soil,standard soil and actual soil,were selected to prepare soil samples with PAHs.An LED-induced fluorescence detection system mainly comprises an excitation light source,sample pool,optical fiber spectrometer,fluorescence signal acquisition device,and computer control unit.The UV LED array beam irradiat

关 键 词：土壤 多环芳烃 诱导荧光光谱 紫外发光二极管 定量检测 

分 类 号：X53[环境科学与工程—环境工程]