IVF-ET对子代胎盘PEG3、L3MBTL1、NF-κB表达的影响及临床意义  

Effects of assisted reproductive technology for treating women on their expression levels of paternally expressed gene 3,paternally imprinted gene L3MBTL1,and NF-κB in placenta tissues and their significances

作　　者：朱珍 高海燕[1] 董自江[1] 李艳[1] 陆薇[1] ZHU Zhen;GAO Haiyan;DONG Zijiang;LI Yan;LU Wei(The First People's Hospital of Lianyungang,Lianyungang,Jiangsu Province,222000)

机构地区：[1]江苏省连云港市第一人民医院,222000

出　　处：《中国计划生育学杂志》2023年第10期2311-2316,共6页Chinese Journal of Family Planning

摘　　要：目的:观察体外受精-胚胎移植(IVF-ET)对子代胎盘父系表达基因3(PEG3)、父系印记基因L3MBTL1(L3MBTL1)、核转录因子-κB(NF-κB)表达水平影响及其临床意义。方法:选取2021年6月-2022年12月在本院产科行IVF-ET的剖宫产产妇及自然受孕剖宫产产妇各45例为IVF-ET组、对照组。胎盘娩出后收集两组胎盘组织,应用实时荧光定量聚合酶链反应(qRT-PCR)、Western blot法测定胎盘组织中PEG3、L3MBTL1、NF-κB mRNA及蛋白表达;比较不同新生儿结局IVF-ET产妇胎盘组织中PEG3、L3MBTL1、NF-κB mRNA及蛋白表达;应用Spearman相关系数分析胎盘组织中各指标表达与新生儿结局关系。结果:IVF-ET组胎盘组织中PEG3、L3MBTL1 mRNA及蛋白表达均低于对照组,NF-κB mRNA及蛋白表达高于对照组;IVF-ET组中出现不良新生儿结局产妇胎盘组织中PEG3、L3MBTL1 mRNA及蛋白表达均低于无不良新生儿结局产妇,NF-κB mRNA及蛋白表达高于无不良新生儿结局产妇(均P<0.05)。Spearman相关系数分析显示,IVF-ET产妇胎盘组织中PEG3、L3MBTL1 mRNA及蛋白表达与新生儿结局呈正相关,NF-κB mRNA及蛋白表达与新生儿结局呈负相关(均P<0.05)。结论:IVF-ET子代胎盘PEG3、L3MBTL1、NF-κB异常表达,且表达与不良新生儿结局有关。Objective:To observe the effects of assisted reproductive technology(IVF-ET) for treating women on their expression levels of paternally expressed gene 3(PEG3),paternally imprinted gene L3MBTL1(L3MBTL1),and nuclear transcription factor-κB(NF-κB) in placenta tissues,and to study their clinical significances.Methods:From June 2021 to December 2022,45 women with cesarean section who had undergone IVF-ET were selected in study group,and 45 women with cesarean section after natural conception during the same period were selected in control group.The placental tissues of the women in the two groups were collected after delivery.The mRNA and protein expressions of PEG3,L3MBTL1,and NF-κB in placental tissues of the women in the two groups were determined by real-time fluorescence quantitative polymerase chain reaction(qRT-PCR) and Western blot.The mRNA and protein expressions of PEG3,L3MBTL1,and NF-κB in placenta tissues were compared among the women with different neonatal outcomes in the study group.Spearman correlation coefficient was used to analyze the correlation between the mRNA and protein expressions of PEG3,L3MBTL1,and NF-κB in placenta tissues of the women and their neonatal outcomes.Results:The mRNA and protein expressions of PEG3 and L3MBTL1 in placenta tissues of the women in the study group were significantly lower than those in placenta tissues of the women in the control group,while the mRNA and protein expression of NF-κB of the women in the study group was significantly higher than that in placenta tissues of the women in control group.In the study group,the mRNA and protein expressions of PEG3 and L3MBTL1 in placenta tissues of the women with adverse neonatal outcomes were significantly lower than those of women with normal neonatal outcomes,and the mRNA and protein expressions of NF-κB in placenta tissues of the women with adverse neonatal outcomes was significantly higher than that of women with normal neonatal outcomes(all P<0.05).Spearman correlation coefficient analysis showed that the mRN

关 键 词：体外受精-胚胎移植 胎盘组织 父系表达基因3 L3MBTL1 核转录因子-ΚB 新生儿结局 相关性 

分 类 号：R714.8[医药卫生—妇产科学]