复发性流产患者抗精子抗体水平与T17/Treg及IL-10/IL-17关系  

Correlation between the antisperm antibody level of patients with recurrent spontaneous abortion and their T helper type 17 cells /regulatory T cells and interleukin 10 /interleukin 17

作　　者：葛亮[1] 张利[1] 杨婉薇[1] 马小力 陶正棋 章雪 GE Liang;ZHANG Li;YANG Wanwei;MA Xiaoli;TAO Zhengqi;ZHANG Xue(Jiangsu Integrated Traditional Chinese and Western Medicine Hospital,Nanjing,Jiangsu Province,210028;Nanjing Maternal and Child Health Care Hospital,Jiangsu Province)

机构地区：[1]江苏省中西医结合医院,南京210028 [2]江苏省南京市妇幼保健院

出　　处：《中国计划生育学杂志》2023年第10期2409-2412,共4页Chinese Journal of Family Planning

摘　　要：目的:分析复发性流产(RSA)患者抗精子抗体(AsAb)水平与辅助T型17细胞(Th17)/调节性T细胞(Treg)及白细胞介素(IL-10)/IL-17关系。方法:收集2022年1-12月于本院就诊的122例复发性流产患者为研究对象,53例女性RSA患者,69例配偶有RSA病史的男性患者;无不良孕产史已生育健康女性40例和配偶无RSA史男性55例作为对照组。检测纳入对象AsAb水平,Th17、Treg细胞数及IL-10、IL-17水平;采用Spearman相关性法分析AsAb阳性表达与Th17、Treg、IL-10、IL-17水平关系。结果:RSA组AsAb阳性8例,其中女性2例、男性6例,对照组阳性仅1例男性;RSA组AsAb阳性率(6.6%)高于对照组(1.1%);RSA组Th17(3.29±1.04个)、Th17/Treg(0.82±0.41)高于对照组(2.01±0.65个、0.29±0.46),Treg(4.02±1.34个)低于对照组(6.85±1.59个);IL-10(399.25±67.96 ng/ml)、IL-10/IL-17(1.34±0.25)低于对照组(602.09±54.31 ng/ml、2.96±0.38),IL-17(298.17±33.54 pg/ml)高于对照组(203.11±27.60 pg/ml);经Spearman法分析,AsAb阳性表达与Th17、Th17/Treg、IL-17水平呈正相关,与Treg、IL-10、IL-10/IL-17水平呈负相关(均P<0.05)。结论:RSA患者AsAb阳性率较高,且与Th17/Treg细胞及IL-10/IL-17相关。Objective:To explore the correlation between the antisperm antibody(AsAb) level of patients with recurrent spontaneous abortion(RSA) and their T helper type 17 cells(TH17)/regulatory T cells(Treg) and interleukin(IL) 10/IL17.Methods:112 patients including 53 women with RSA and 69 men with RSA history of their spouses were enrolled in study group,and 40 healthy women with child and without adverse pregnancy and birth history and the spouses of 55 men without RSA history were enrolled in control group from January to December 2022.The AsAb level,the numbers of Th17 and Treg cells,and the levels of IL10 and IL17 of the patients in the two groups were detected.Spearman correlation method was used to analyze the correlation between the AsAb positive expression of the patients and their Th17 and Treg cells numbers and their IL10 and IL17 levels.Results:There were 8 patients with positive AsAb in the study group,including 2 women and 6 men,but there was only 1 case with positive AsAb in the control group.The positive rate of AsAb(6.6%) of the patients in the study group was significantly higher than that(1.1%) of the patients in the control group.The values of Th17(3.29±1.04) and Th17/Treg(0.82±0.41) of the patients in the study group were significantly higher than those(2.01±0.65 and 0.29±0.46) of the patients in the control group,and the number of Treg cells(4.02±1.34) of the patients in the study group was significantly lower than that(6.85±1.59) of the patients in the control group.The IL10 level(399.25±67.96ng/ml) and the IL10/IL17 value(1.34±0.25)of the patients in the study group were significantly lower than those(602.09±54.31ng/ml and 2.96±0.38)of the patients in the control group.The IL17level(298.17±33.54pg/ml)of the patients in the study group was significantly higher than that(203.11±27.60pg/ml)of the patients in the control group.Spearman analysis showed that the AsAb positive expression of the patients was positively correlated with their Th17and IL17levels,and their Th17/Treg value,but was ne

关 键 词：复发性流产 抗精子抗体 辅助性T型17细胞 调节性T细胞 白细胞介素10 白细胞介素17 相关性 

分 类 号：R714.21[医药卫生—妇产科学]