淫羊藿诱导ADSCs成骨分化作用于骨质疏松症防治的研究进展  

Research Progress on the osteogenic differentiation of ADSCs induced by epimedium in the prevention and treatment of osteoporosis

作　　者：刘海全[1,2] 吴梅剑宗 蒙祖玉 Hai-Quan LIU;Mei-Jian-Zong WU;Zu-Yu MENG(Department of Orthopaedics,Huizhou Hospital of Guangzhou University of Chinese Medicine,Huizhou 516000,Guangdong Province,China;Department of Orthopaedics,Huizhou Hospital of Traditional Chinese Medicine,Huizhou 516000,Guangdong Province,China;School of Traditional Chinese Medicine,Jinan University,Guangzhou 510000,China)

机构地区：[1]广州中医药大学惠州医院骨科,广东惠州516000 [2]惠州市中医医院骨科,广东惠州516000 [3]暨南大学中医学院,广州510000

出　　处：《医学新知》2023年第5期381-388,共8页New Medicine

基　　金：国家自然科学基金面上项目(82174147);广东省基础与应用基础研究基金项目(2021A1515011271,2023A515011686);广东省中医药局科研项目(20213019)。

摘　　要：骨质疏松症(osteoporosis,OP)是一种慢性、全身性骨病,伴随着老龄化社会的到来,对于该疾病防治的研究日益增多,寻找有效药物诱导脂肪间充质干细胞(adipose-derived mesenchymal stem cells,ADSCs)成骨分化是目前研究较为热点的OP防治策略。过氧化物酶体增殖物激活受体γ(peroxisome proliferator-activated receptor gamma,PPARγ)为调控脂肪细胞分化的关键蛋白,研究证实应用淫羊藿干预骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMSCs),能抑制PPARγ表达,从而抑制BMSCs成脂分化,促进其成骨分化。Hippo信号通路通过激酶级联控制YAP/TAZ转录辅活化因子的活性,抑制PPARγ活性,进而抑制ADSCs向脂肪细胞分化。Ajuba是细胞成脂分化的重要调节剂,是一种新型的PPARγ相互作用蛋白,在细胞增殖过程中能够限制Hippo信号通路活性。中药淫羊藿在OP防治方面具有独特的优势,前期研究发现淫羊藿可促进ADSCs成骨分化,抑制其成脂分化。在OP发病机制中,PPARγ、Hippo通路、Ajuba三者之间存在着密切的关联性。结合前期实验研究,本文旨在对中药淫羊藿作用于ADSCs防治OP的作用机制进行综述,以期为临床新药研发提供参考。Osteoporosis(OP)is a chronic,systemic bone disease.With the arrival of the aging society,the research on the prevention and treatment of this disease is increasing.Finding effective drugs to induce osteogenesis and differentiation of adipose-derived mesenchymal stem cells(ADSCs)is currently a more focused strategy for prevention and treatment of OP.Peroxisome proliferator-activated receptor gamma(PPARγ)is the key protein of adipocyte differentiation.Studies have confirmed that the intervention of epimedium on bone marrow mesenchymal stem cells(BMSCs)can inhibit the expression of PPARγ,thus inhibits adipogenic differentiation of BMSCs and promotes their osteogenic differentiation.Hippo signal pathway controls the activity of YAP/TAZ transcriptional coactivator through kinase cascade and inhibiting PPARγ,which can inhibit ADSCs from differentiating into adipocytes.Ajuba is an important regulator of adipogenic differentiation of cells and a new PPARγinteracting proteins which can limit the activity of Hippo signaling pathway during cell proliferation.Epimedium has unique advantages in the prevention and treatment of OP.Previous studies found that epimedium can promote the osteogenic differentiation of ADSCs and inhibit their lipogenic differentiation.PPARγ,Hippo pathway and Ajuba are closely related in the pathogenesis of OP.According to previous research,this paper is purposed to review the mechanism of epimedium in ADSCs for the prevention and treatment of OP,and to provide a reference for the development of new clinical drugs.

关 键 词：淫羊藿 骨质疏松 脂肪间充质干细胞 成骨分化 PPARγ/Hippo/Ajuba通路 

分 类 号：R259[医药卫生—中西医结合]